International Genome Centre, Jiangsu University, Zhenjiang, 212013, China.
State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, 210008, China.
Environ Microbiol. 2021 Feb;23(2):1130-1144. doi: 10.1111/1462-2920.15261. Epub 2020 Oct 15.
Microbes play an important role in decomposition of macrophytes in shallow lakes, and the process can be greatly affected by bacteria-fungi interactions in response to material composition and environmental conditions. In this study, microbes involved in the decomposition of leaf litter from three macrophyte species, Zizania latifolia, Hydrilla verticillata and Nymphoides peltata, were analysed at temperatures of 5, 15 and 25 °C. Results indicate that the decomposition rate was affected by temperature. Bacterial alpha diversity increased significantly along the time, while both temperature and plant species had a significant impact on the bacterial community, and plant type was shown to be the most important driving factor for the fungal community. The cosmopolitan bacterial taxa affiliated with Gammaproteobacteria, Bacteroidetes, Deltaproteobacteria, Firmicutes and Spirochaetes were key species in the investigated ecological networks, demonstrating significant co-occurrence or co-exclusion relationships with Basidiomycota and Ascomycota, according to different macrophyte species. This study indicates that bacteria involved in the decomposition of macrophyte leaf litter are more sensitive to temperature variance, and that fungi have a higher specificity to the composition of plant materials. The nutrient content of Hydrilla verticillata promoted a positive bacteria-fungi interaction, thereby accelerating the decomposition and re-circulation of leaf litter.
微生物在浅水湖泊中大型水生植物的分解过程中起着重要作用，而细菌-真菌相互作用对物质组成和环境条件的响应会极大地影响这一过程。本研究在 5、15 和 25°C 的温度下，分析了三种大型水生植物（菰、黑藻和紫萍）叶片凋落物分解过程中涉及的微生物。结果表明，分解速率受温度的影响。细菌的 α 多样性随着时间的推移显著增加，而温度和植物种类对细菌群落均有显著影响，且植物类型是真菌群落的最重要驱动因素。与广布细菌类群γ变形菌门、拟杆菌门、δ 变形菌门、厚壁菌门和螺旋体门相关的细菌是调查生态网络中的关键种，与担子菌门和子囊菌门表现出显著的共同出现或共同排斥关系，这与不同的大型水生植物种类有关。本研究表明，参与大型水生植物叶片凋落物分解的细菌对温度变化更为敏感，而真菌对植物材料的组成具有更高的特异性。黑藻的营养含量促进了细菌-真菌的正相互作用，从而加速了叶片凋落物的分解和再循环。
