Analysis of virulence traits of isolated from bovine mastitis in semi-intensive and family dairy farms.

BACKGROUND

is one of the main microorganisms that causes bovine mastitis, and its well-known virulence characteristics and interactions with the environment are used to aid the design of more efficient therapies.

OBJECTIVES

To determine whether the virulence traits, such as antibiotic resistance and biofilm-forming and internalization abilities, of isolated from bovine mastitis are related to dairy production system types.

METHODS

The study was performed in the Mexican states of Guanajuato and Michoacan. Semi-intensive dairy farms (SIDFs) and family dairy farms (FDFs) (454 and 363 cows, respectively) were included. The 194 milk samples from mastitis affected quarters were collected and 92 strains of were isolated and identified by biochemical and molecular tests. Antibiotic resistance, biofilm and internalization assays were performed on 30 randomly selected isolated strains to determine virulence traits, and these strains were equally allocated to the 2 dairy production systems.

RESULTS

All 30 selected strains displayed a high degree of resistance (50%-91.7%) to the antibiotics tested, but no significant difference was found between SIDF and FDF isolates. strains from SIDFs had an average biofilm forming capacity of up to 36% (18.9%-53.1%), while strains from FDFs registered an average of up to 53% (31.5%-77.8%) ( > 0.05). Internalization assays revealed a higher frequency of internalization capacity for strains isolated from FDFs (33.3%) than for those isolated from SIDFs (6.7%) ( > 0.05). gen was detected in 46.6% of FDF strains and 33.3% of SIDF strains, and this difference was significant ( < 0.05).

CONCLUSIONS

Our findings show that the virulence traits of isolates analyzed in this study, depend significantly on several factors, such as phenotype, genotype, and environmental conditions, which are significantly related to dairy production system type and daily management practices.