Kaduk B, Husslein E M, Siegfried A
Hepatogastroenterology. 1987 Jun;34(3):108-12.
Male Wistar rats were fed Busulfan dissolved in fat milk at a daily dose of 0.35, 0.89, 1.20 mg/kg body weight for a period of four, eight and twelve weeks. Examination by light microscopy, including immunohistochemistry, and by electron microscopy revealed changes in the pancreatic islets which were dependent on dose and time. First, hyperaemia was demonstrated, then perivascular "inbleedings" were followed by organisation and islet fibrosis with centripetal reduction of the islet cells. This resulted in an almost complete loss of A-cells and a reduction in the number of B-cells. A morphological factor analysis suggested additional analogies to type I - diabetes mellitus, so that for the formal pathogenesis, islet haematoma and immunological events have to be discussed.
将雄性Wistar大鼠用溶解于脂肪乳中的白消安以每日0.35、0.89、1.20mg/kg体重的剂量喂养4周、8周和12周。通过光学显微镜检查（包括免疫组织化学）和电子显微镜检查发现，胰岛的变化取决于剂量和时间。首先出现充血，随后是血管周围“出血”，接着是机化和胰岛纤维化，胰岛细胞向心性减少。这导致A细胞几乎完全丧失，B细胞数量减少。形态学因素分析表明与I型糖尿病还有其他相似之处，因此就形式发病机制而言，必须讨论胰岛血肿和免疫事件。
