São Leopoldo Mandic, Laboratory of Neuroimmune Interface of Pain Research, Campinas, Brazil.
Faculty of Pharmaceutical Sciences (FCF), University of Campinas (UNICAMP), Campinas, Brazil.
Nat Prod Res. 2021 Dec;35(24):6106-6111. doi: 10.1080/14786419.2020.1826482. Epub 2020 Oct 4.
This study evaluated the phytochemical characterization of (BO extract) unsaponifiable extract and resulting fractions (F fraction - FF, Geranyl fraction - GF and R fraction- RF) obtained as by-products of an industrial process investigating antiproliferative activities in human tumoral cells. The main compounds identified by GC-MS for BO extract were Geranylgeraniol (61.51%); for FF: Geranylgeraniol (70.23%); for GF: Geranylgeraniol (78.92%) and for RF: -cubebene (27.75%). Quantifications of geranylgeraniol by GC-FID presented the percentage content: BO 27.52%; FF 38.52%; GF 51.44% and RF 1.81%. BO extract showed a significant antiproliferative activity, with GI up to 4 µg/mL. All fractions had a remarkably similar antiproliferative activity profile (GI 27-47 µg/mL). Data reported herein showed an important cytostatic effect for BO extract, nevertheless this activity is not attributed exclusively to geranylgeraniol. In conclusion, this by-product becomes of great value, being a potential candidate for development of new anti-tumor ingredients.
本研究评估了 （BO 提取物）不皂化物提取物和所得馏分（F 馏分 - FF、香叶基馏分 - GF 和 R 馏分 - RF）的植物化学特征，这些馏分是工业过程的副产品，用于研究人类肿瘤细胞的抗增殖活性。GC-MS 鉴定的 BO 提取物的主要化合物为香叶二烯醇（61.51%）；FF：香叶二烯醇（70.23%）；GF：香叶二烯醇（78.92%）和 RF：-古巴烯（27.75%）。GC-FID 对香叶二烯醇的定量分析结果显示，BO 的含量为 27.52%；FF 为 38.52%；GF 为 51.44%，RF 为 1.81%。BO 提取物表现出显著的抗增殖活性，GI 高达 4μg/mL。所有馏分的抗增殖活性谱（GI 27-47μg/mL）非常相似。本文报道的数据显示 BO 提取物具有重要的细胞生长抑制作用，但这种活性并非仅归因于香叶二烯醇。总之，这种副产品具有很高的价值，可能成为开发新抗肿瘤成分的候选物。
