工业过程中的 L. 副产物馏分：对肿瘤细胞的抗增殖活性和化学特征。

L. by-products' fractions from an industrial process: antiproliferative activity on tumor cells and chemical profile.

机构信息

São Leopoldo Mandic, Laboratory of Neuroimmune Interface of Pain Research, Campinas, Brazil.

Faculty of Pharmaceutical Sciences (FCF), University of Campinas (UNICAMP), Campinas, Brazil.

出版信息

Nat Prod Res. 2021 Dec;35(24):6106-6111. doi: 10.1080/14786419.2020.1826482. Epub 2020 Oct 4.

DOI:10.1080/14786419.2020.1826482

PMID:33016138

Abstract

This study evaluated the phytochemical characterization of (BO extract) unsaponifiable extract and resulting fractions (F fraction - FF, Geranyl fraction - GF and R fraction- RF) obtained as by-products of an industrial process investigating antiproliferative activities in human tumoral cells. The main compounds identified by GC-MS for BO extract were Geranylgeraniol (61.51%); for FF: Geranylgeraniol (70.23%); for GF: Geranylgeraniol (78.92%) and for RF: -cubebene (27.75%). Quantifications of geranylgeraniol by GC-FID presented the percentage content: BO 27.52%; FF 38.52%; GF 51.44% and RF 1.81%. BO extract showed a significant antiproliferative activity, with GI up to 4 µg/mL. All fractions had a remarkably similar antiproliferative activity profile (GI 27-47 µg/mL). Data reported herein showed an important cytostatic effect for BO extract, nevertheless this activity is not attributed exclusively to geranylgeraniol. In conclusion, this by-product becomes of great value, being a potential candidate for development of new anti-tumor ingredients.

摘要

本研究评估了（BO 提取物）不皂化物提取物和所得馏分（F 馏分 - FF、香叶基馏分 - GF 和 R 馏分 - RF）的植物化学特征，这些馏分是工业过程的副产品，用于研究人类肿瘤细胞的抗增殖活性。GC-MS 鉴定的 BO 提取物的主要化合物为香叶二烯醇（61.51%）；FF：香叶二烯醇（70.23%）；GF：香叶二烯醇（78.92%）和 RF：-古巴烯（27.75%）。GC-FID 对香叶二烯醇的定量分析结果显示，BO 的含量为 27.52%；FF 为 38.52%；GF 为 51.44%，RF 为 1.81%。BO 提取物表现出显著的抗增殖活性，GI 高达 4μg/mL。所有馏分的抗增殖活性谱（GI 27-47μg/mL）非常相似。本文报道的数据显示 BO 提取物具有重要的细胞生长抑制作用，但这种活性并非仅归因于香叶二烯醇。总之，这种副产品具有很高的价值，可能成为开发新抗肿瘤成分的候选物。