Medicinal Plant Biotechnology Lab, Department of Botany, University of Delhi, Delhi, India.
Artif Cells Nanomed Biotechnol. 2020 Dec;48(1):1259-1271. doi: 10.1080/21691401.2020.1817056.
The present study encompasses green synthesis of silver nanoparticles (AgNPs) using aqueous leaf extract of Arabian Primrose within 6 min of reaction at 60 °C, pH 7 and their characterisation using physico-chemical analytical techniques. UV-Visible spectroscopy elucidated the surface plasmon resonance around 420 nm. FESEM and TEM images revealed that AgNPs were spherical with average diameter 10-60 nm. XRD pattern confirmed their crystalline nature. The leaf extract rich in phenolics and flavonoids was subjected to GC-MS analysis that identified bioactive compounds helping in reduction and stabilisation of AgNPs. The synthesised AgNPs possessed high anti-oxidant potential against DPPH and HO radicals. Incidentally, the AgNPs acted as excellent nanocatalyst towards borohydride reduction and degradation of structurally different organic dyes. The AgNPs also exhibited selective colorimetric sensing of hazardous mercuric, ferric ions and ammonia. Such AgNPs were cytotoxic against HeLa cells (IC 7.18 µg/mL) and compatible towards normal LB cells. These AgNPs showed effective anti-microbial activity against different human pathogens tested (bacterial and fungal). This is probably the first report of AgNPs synthesis using Arabian Primrose leaf extract showing strong anti-oxidant, catalytic, biosensing, anti-cancer and anti-microbial activities and find remarkable applications in medical, industrial and ecological sectors.
本研究采用阿拉伯月见草叶的水提液，在 60°C、pH 值为 7 的条件下，于 6 分钟内实现了银纳米粒子（AgNPs）的绿色合成，并采用物理化学分析技术对其进行了表征。紫外-可见光谱表明其表面等离子体共振在 420nm 左右。FESEM 和 TEM 图像显示 AgNPs 呈球形，平均直径为 10-60nm。XRD 图谱证实了其结晶性质。叶提取物富含酚类和类黄酮，通过 GC-MS 分析鉴定出有助于 AgNPs 还原和稳定的生物活性化合物。合成的 AgNPs 具有较高的抗氧化能力，可有效清除 DPPH 和 HO 自由基。此外，AgNPs 还可作为高效纳米催化剂，用于硼氢化还原和不同结构的有机染料降解。AgNPs 还对 Hg2+、Fe3+和氨等有害物质表现出选择性比色传感。这些 AgNPs 对 HeLa 细胞具有细胞毒性（IC7.18µg/mL），对正常 LB 细胞具有相容性。AgNPs 对测试的不同人类病原体（细菌和真菌）具有有效的抗菌活性。这可能是首次报道使用阿拉伯月见草叶提取物合成 AgNPs，其具有较强的抗氧化、催化、生物传感、抗癌和抗菌活性，并在医学、工业和生态领域得到了广泛应用。
