Department of Cardiovascular Medicine, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China.
Department of Anesthesiology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Perfusion. 2021 Oct;36(7):717-723. doi: 10.1177/0267659120960306. Epub 2020 Oct 4.
Previous studies have pointed out that sevoflurane (Sef) preconditioning could relieve myocardial ischemia/reperfusion (I/R) injury, but the mechanisms is still unknown.
C57BL/6 mice model of myocardial I/R injury was established to evaluate the function of Sef. Briefly, Sef was inhaled before I/R operation. The levels of TIMP3, oxidative damage-related factors, and mitogen activated protein kinases (MAPKs) pathway-related factors were measured by qRT-PCR and western blot. Myocardial infarction (MI) area was detected by triphenyl tetrazolium chloride (TTC) staining assay.
Sef preconditioning reduced MI area in myocardial I/R injury mice and upregulated TIMP3 expression in myocardial tissues of I/R mice. In addition, downregulation of TIMP3 reversed the alleviating effects of Sef pretreatment on myocardial oxidative damage and inhibited the effect of Sef pretreatment on MAPKs pathway activity.
Sef preconditioning ameliorated myocardial I/R injury by modulating MAPKs pathway activity via upregulating TIMP3.
先前的研究指出，七氟醚（Sef）预处理可以减轻心肌缺血/再灌注（I/R）损伤，但具体机制尚不清楚。
建立 C57BL/6 小鼠心肌 I/R 损伤模型，以评估 Sef 的功能。简要来说，在 I/R 手术前吸入 Sef。通过 qRT-PCR 和 Western blot 检测 TIMP3、氧化损伤相关因子和丝裂原活化蛋白激酶（MAPKs）途径相关因子的水平。通过氯化三苯基四氮唑（TTC）染色检测心肌梗死（MI）面积。
Sef 预处理可减少心肌 I/R 损伤小鼠的 MI 面积，并上调 I/R 小鼠心肌组织中的 TIMP3 表达。此外，下调 TIMP3 可逆转 Sef 预处理对心肌氧化损伤的缓解作用，并抑制 Sef 预处理对 MAPKs 途径活性的作用。
Sef 预处理通过上调 TIMP3 调节 MAPKs 途径活性，改善心肌 I/R 损伤。
