College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi, 541004, China.
Analyst. 2021 Jan 7;145(24):7893-7897. doi: 10.1039/d0an01606c.
MicroRNAs (miRNAs) have played a vital role in the regulation of gene expression and have been considered as potential biomarker candidates for early cancer diagnosis. Rapid and sensitive detection of microRNAs is highly desired. Here, we present a new method to rapidly and sensitively determine microRNAs based on the technology of gold nanoparticle catalyzed silver staining enhancement. The new method involves the sandwich hybridization of a capture probe immobilized on a magnetic bead, a reporter probe assembled on gold nanoparticles and a miRNA target, catalytic silver precipitation by gold nanoparticles, magnetic collection of the enhanced sandwich complex, dissolution of the silver precipitation and stripping detection. Using the proposed method the microRNA-7a assay was successfully carried out in less than 70 min and the detection limit was as low as 15 fM. The proposed biosensor may hold great promise in biological monitoring of microRNAs.
微小 RNA（miRNAs）在基因表达调控中发挥着重要作用，被认为是癌症早期诊断的潜在生物标志物候选者。快速灵敏地检测微小 RNA 是人们所期望的。在这里，我们提出了一种新的基于金纳米粒子催化银染增强技术快速灵敏地测定微小 RNA 的方法。该方法涉及到捕获探针固定在磁性珠上的夹心杂交、组装在金纳米粒子上的报告探针和微小 RNA 靶标、金纳米粒子催化银沉淀、增强夹心复合物的磁性收集、银沉淀的溶解和剥离检测。利用该方法，在不到 70 分钟的时间内成功地进行了 microRNA-7a 的测定，检测限低至 15 fM。该生物传感器在微小 RNA 的生物监测中可能具有广阔的应用前景。
