Emergency Center, Bayannur Hospital, Bayannur, China.
Department of Stomatology, Children's Hospital of Nanjing Medical University, Nanjing, China.
Histol Histopathol. 2020 Nov;35(11):1285-1293. doi: 10.14670/HH-18-267. Epub 2020 Oct 5.
The nuclear pore membrane protein 121 (POM121) is an important member of the nuclear pore complex which regulates nucleocytoplasmic transport, but little is known about the role of POM121 in laryngeal cancer. In this study, quantitative real-time polymerase chain reaction and immunohistochemistry were performed to detect POM121 expression in laryngeal tissues. The associations between POM121 and clinicopathological characteristics and overall survival in laryngocarcinoma patients were also analyzed. The mechanism of POM121 was preliminarily explored through gene set enrichment analysis (GSEA). mRNA and protein expression of POM121 in laryngocarcinoma tissues were higher than those in nontumor tissues. High POM121 expression was positively correlated with poor differentiation (χ²=42.391, P<0.001), advanced distant metastases (χ²=20.346, P<0.001) and TNM stage (χ²=23.436, P<0.001). Laryngocarcinoma patients with high POM121 level tended to have poor overall survival. GSEA confirmed that the mechanism of POM121 in laryngeal cancer may relate to sphingolipid metabolism, lysosome, fatty acid metabolism, ribosome, nucleotide excision repair and the PPAR signaling pathway. Overall, POM121 expression might be a prognostic biomarker in laryngeal cancer, and POM121 has the potential to present as a therapeutic target for laryngocarcinoma patients.
核孔膜蛋白 121（POM121）是核孔复合物的重要成员，调节核质转运，但关于 POM121 在喉癌中的作用知之甚少。本研究通过实时定量聚合酶链反应和免疫组织化学检测了喉组织中 POM121 的表达。还分析了 POM121 与喉癌患者临床病理特征和总生存的关系。通过基因集富集分析（GSEA）初步探讨了 POM121 的作用机制。喉癌组织中 POM121 的 mRNA 和蛋白表达均高于非肿瘤组织。高 POM121 表达与低分化（χ²=42.391，P<0.001）、晚期远处转移（χ²=20.346，P<0.001）和 TNM 分期（χ²=23.436，P<0.001）呈正相关。POM121 水平高的喉癌患者总生存较差。GSEA 证实 POM121 在喉癌中的作用机制可能与鞘脂代谢、溶酶体、脂肪酸代谢、核糖体、核苷酸切除修复和 PPAR 信号通路有关。总之，POM121 的表达可能是喉癌的预后生物标志物，POM121 有可能成为喉癌患者的治疗靶点。
