College of Pharmacy, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China.
Thorac Cancer. 2020 Nov;11(11):3365-3374. doi: 10.1111/1759-7714.13682. Epub 2020 Oct 5.
One classic traditional Chinese medicine theory is that the "lung and intestine are exterior-interiorly related"; however, this has not been confirmed experimentally. The aim of this study was to provide a biological basis for the theory by measuring the tissue distribution of andrographolide.
Acute pneumonia was induced in a mouse model by repeated stimulation with lipopolysaccharide. The distribution of andrographolide in mice was observed by positron emission tomography (PET) imaging with [ F]-labeled andrographolide, and changes in the in vivo distribution before and after modeling were compared. Subsequently, the consistency of pathological changes in lung and intestine was confirmed by observation of pathological sections. Finally, the results were verified by cytokine detection.
The value of organ uptake, pathological changes and inflammatory factor expression of the lung and intestine were consistent. The concentration of andrographolide in the lung and intestine increased significantly, and was confirmed by pathology and enzyme-linked immunosorbent assays (ELISA).
Micro-positron emission tomography (microPET) can be used to visually observe the distribution of medicinal ingredients in vivo, and [ F]-andrographolide can be used as a tool to assess the interior-exterior relationship between the lung and intestine.
中医的经典理论之一是“肺与大肠相表里”，但这尚未得到实验证实。本研究旨在通过测量穿心莲内酯的组织分布，为该理论提供生物学依据。
通过重复脂多糖刺激建立小鼠急性肺炎模型，用[ F]-标记的穿心莲内酯进行正电子发射断层扫描（PET）成像，观察穿心莲内酯在小鼠体内的分布，并比较建模前后体内分布的变化。随后，通过观察病理切片证实肺和肠的病理变化的一致性。最后，通过细胞因子检测验证结果。
肺和肠的器官摄取值、病理变化和炎症因子表达一致。肺和肠组织中穿心莲内酯的浓度显著增加，这通过病理学和酶联免疫吸附试验（ELISA）得到了证实。
微正电子发射断层扫描（microPET）可用于直观观察药物成分在体内的分布，[ F]-穿心莲内酯可作为评估肺与肠表里关系的工具。
