Bogema Daniel R, Yam Jerald, Micallef Melinda L, Gholipourkanani Hosna, Go Jeffrey, Jenkins Cheryl, Dang Cecile
Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2568, Australia.
Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2568, Australia; The Ithree Institute, University of Technology Sydney, NSW 2007, Australia.
Genomics. 2021 Jan;113(1 Pt 2):677-688. doi: 10.1016/j.ygeno.2020.09.064. Epub 2020 Oct 2.
Perkinsus spp. parasites have significant impact on aquaculture and wild mollusc populations. We sequenced the genomes of five monoclonal isolates of Perkinsus olseni and one Perkinsus chesapeaki from international sources. Sequence analysis revealed similar levels of repetitive sequence within species, a polyploid genome structure, and substantially higher heterozygosity in Oceanian-sourced isolates. We also identified tandem replication of the rRNA transcriptional unit, with high strain variation. Characterized gene content was broadly similar amongst all Perkinsus spp. but P. olseni Oceanian isolates contained an elevated number of genes compared to other P. olseni isolates and cox3 could not be identified in any Perkinsus spp. sequence. Phylogenetics and average nucleotide identity scans were consistent with all P. olseni isolates being within one species. These are the first genome sequences generated for both P. olseni and P. chesapeaki and will allow future advances in diagnostic design and population genomics of these important aquatic parasites.
派金虫属寄生虫对水产养殖和野生软体动物种群有重大影响。我们对来自国际来源的五个奥尔森派金虫单克隆分离株和一个切萨皮克派金虫的基因组进行了测序。序列分析揭示了物种内相似水平的重复序列、多倍体基因组结构，以及来自大洋洲的分离株中显著更高的杂合性。我们还鉴定出rRNA转录单元的串联重复，且菌株变异程度高。所有派金虫属物种的特征性基因含量大致相似，但与其他奥尔森派金虫分离株相比，来自大洋洲的奥尔森派金虫分离株含有更多的基因，并且在任何派金虫属物种序列中均未鉴定出细胞色素c氧化酶亚基3（cox3）。系统发育学和平均核苷酸同一性扫描结果表明，所有奥尔森派金虫分离株都属于同一个物种。这些是首次为奥尔森派金虫和切萨皮克派金虫生成的基因组序列，将为这些重要水生寄生虫的诊断设计和群体基因组学的未来发展提供支持。
