College of Life Science, Dalian Nationalities University, Dalian, 116600, PR China.
College of Life Science, Dalian Nationalities University, Dalian, 116600, PR China; School of Microelectronics, Dalian University of Technology, Dalian, 116024, PR China.
Colloids Surf B Biointerfaces. 2021 Jan;197:111379. doi: 10.1016/j.colsurfb.2020.111379. Epub 2020 Sep 28.
The probing and quantitative detection of nitroaromatics is key for public safety and the monitoring of wastewater. Currently, most techniques used for the detection of nitroaromatics require ideal conditions rather than real conditions, making practical applications challenging. As nitroaromatics have strong absorption in the range of 350-370 nm, we can design a kind of KMgF:Eu nanophosphor with a strong f-f transition emission located at 362 nm, and an energy resonance transfer system based on the overlap of the emission peak of nanophosphors and the absorption peak of nitroaromatics can be constructed to realize the quantitative detection of nitroaromatics in municipal wastewater. Based on this, in this paper, a fluorescence resonance energy transfer (FRET) sensor is designed by choosing polyethylenimine (PEI)-capped KMgF:Eu nanoparticles as an energy donor for the ultrasensitive detection of nitroaromatics, which can also work as an energy acceptor. The KMgF:Eu nanoparticle sensor shows great sensitivity and selectivity and good linear characteristics in both DI water and wastewater. The detection limits in municipal wastewater were 0.456, 0.598, 0.667, 0557 and 0.678 ng/mL for TNT, TNP, p-nitrotoluene, dinitrobenzene (DNB), and nitrobenzene (NB), respectively. The detection accuracy was identified by high-performance liquid chromatography (HPLC). The results showed that the sensor had superior sensitivity and great accuracy and could be used in practical applications.
硝基芳烃的探测和定量检测对于公共安全和废水监测至关重要。目前，大多数用于检测硝基芳烃的技术都需要理想条件，而不是实际条件，这使得实际应用具有挑战性。由于硝基芳烃在 350-370nm 范围内具有很强的吸收，我们可以设计一种具有位于 362nm 的强 f-f 跃迁发射的 KMgF:Eu 纳米荧光粉，并构建基于纳米荧光粉发射峰和硝基芳烃吸收峰重叠的能量共振转移体系，以实现对城市废水中硝基芳烃的定量检测。基于此，在本文中，选择聚乙烯亚胺（PEI）封端的 KMgF:Eu 纳米粒子作为能量供体，设计了一种荧光共振能量转移（FRET）传感器，用于超灵敏检测硝基芳烃，同时也可以作为能量受体。在去离子水和废水中，KMgF:Eu 纳米粒子传感器均表现出了极大的灵敏度和选择性，以及良好的线性特征。在城市废水中，TNT、TNP、对硝基甲苯、二硝基苯（DNB）和硝基苯（NB）的检测限分别为 0.456、0.598、0.667、0.557 和 0.678ng/mL。通过高效液相色谱（HPLC）对检测精度进行了验证。结果表明，该传感器具有较高的灵敏度和准确性，可用于实际应用。
