Single Cell Level Dielectrophoretic Responses & Dielectrophoretic Deformations of Monocytes to Quantify Population Heterogeneity.

Single-cell dielectrophoretic movement and dielectrophoretic deformation of monocyte cells were interrogated applying 20 V, 50 kHz to 1 MHz signal in the 3D carbon electrode array. Heterogeneity of the monocyte population is shown in terms of the crossover frequencies, translational movement, and deformation index of the cells. The results presented that crossover range for monocytes was 100 kHz - 200 kHz, the translational movement of the cells was rapidly altered when the initial positions of the cells were in the negative dielectrophoretic region. Finally, the deformation index of the monocyte population varied from 0.5 to 1.5.