Sengul Esra, Sharbati Pouya, Elitas Meltem, Islam Monsur, Korvink Jan G
Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey.
Sabanci University Nanotechnology Research and Application Center, Istanbul, Turkey.
Electrophoresis. 2022 Jun;43(12):1357-1365. doi: 10.1002/elps.202100374. Epub 2022 Apr 26.
Glioblastoma multiforme is the most aggressive and invasive brain cancer consisting of genetically and phenotypically altering glial cells. It has massive heterogeneity due to its highly complex and dynamic microenvironment. Here, electrophysiological properties of U87 human glioma cell line were measured based on a dielectrophoresis phenomenon to quantify the population heterogeneity of glioma cells. Dielectrophoretic forces were generated using a gold-microelectrode array within a microfluidic channel when 3 V and 100, 200, 300, 400, 500 kHz, 1, 2, 5, and 10 MHz frequencies were applied. We analyzed the dielectrophoretic behavior of 500 glioma cells, and revealed that the crossover frequency of glioma cells was around 140 kHz. A quantifying dielectrophoretic movement of the glioma cells exhibited three distinct glioma subpopulations: 50% of the glioma cells experienced strong, 30% of the cells were spread in the microchannel by moderate, and the rest of the cells experienced very weak positive dielectrophoretic forces. Our results demonstrated the dielectrophoretic spectra of U87 glioma cell line. Dielectrophoretic responses of glioma cells linked population heterogeneity to membrane properties of glioma cells rather than their size distribution in the population.
多形性胶质母细胞瘤是最具侵袭性的脑癌，由基因和表型发生改变的神经胶质细胞组成。由于其高度复杂和动态的微环境，它具有巨大的异质性。在此，基于介电泳现象测量了U87人胶质瘤细胞系的电生理特性，以量化胶质瘤细胞的群体异质性。当施加3V以及100、200、300、400、500kHz、1、2、5和10MHz频率时，使用微流控通道内的金微电极阵列产生介电泳力。我们分析了500个胶质瘤细胞的介电泳行为，发现胶质瘤细胞的交叉频率约为140kHz。对胶质瘤细胞介电泳运动的量化显示出三个不同的胶质瘤亚群：50%的胶质瘤细胞经历强介电泳力，30%的细胞在微通道中以中等介电泳力扩散，其余细胞经历非常弱的正介电泳力。我们的结果展示了U87胶质瘤细胞系的介电泳谱。胶质瘤细胞的介电泳响应将群体异质性与胶质瘤细胞的膜特性联系起来，而不是与它们在群体中的大小分布联系起来。
