帽蛋白Z（36/32）是一种带刺的肌动蛋白封端蛋白，是骨骼肌Z线的一个组成部分。
Cap Z(36/32), a barbed end actin-capping protein, is a component of the Z-line of skeletal muscle.
作者信息
Casella J F, Craig S W, Maack D J, Brown A E
出版信息
J Cell Biol. 1987 Jul;105(1):371-9. doi: 10.1083/jcb.105.1.371.
Abstract
Various biological activities have been attributed to actin-capping proteins based on their in vitro effects on actin filaments. However, there is little direct evidence for their in vivo activities. In this paper, we show that Cap Z(36/32), a barbed end, actin-capping protein isolated from muscle (Casella, J. F., D. J. Maack, and S. Lin, 1986, J. Biol. Chem., 261:10915-10921) is localized to the barbed ends of actin filaments by electron microscopy and to the Z-line of chicken skeletal muscle by indirect immunofluorescence and electron microscopy. Since actin filaments associate with the Z-line at their barbed ends, these findings suggest that Cap Z(36/32) may play a role in regulating length, orienting, or attaching actin filaments to Z-discs.
摘要
基于肌动蛋白封端蛋白对肌动蛋白丝的体外作用，人们赋予了它们多种生物学活性。然而，几乎没有直接证据证明它们在体内的活性。在本文中，我们发现Cap Z(36/32)，一种从肌肉中分离出来的位于肌动蛋白丝尖端的封端蛋白（Casella, J. F., D. J. Maack, and S. Lin, 1986, J. Biol. Chem., 261:10915 - 10921），通过电子显微镜观察定位于肌动蛋白丝的尖端，通过间接免疫荧光和电子显微镜观察定位于鸡骨骼肌的Z线。由于肌动蛋白丝在其尖端与Z线相连，这些发现表明Cap Z(36/32)可能在调节肌动蛋白丝长度、使其定向或将其附着于Z盘方面发挥作用。
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Cap Z(36/32), a barbed end actin-capping protein, is a component of the Z-line of skeletal muscle.帽蛋白Z（36/32）是一种带刺的肌动蛋白封端蛋白，是骨骼肌Z线的一个组成部分。
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本文引用的文献
1
New actin-binding proteins from Dictyostelium discoideum.从盘基网柄菌中提取的新的肌动蛋白结合蛋白。
EMBO J. 1984 Sep;3(9):2095-100. doi: 10.1002/j.1460-2075.1984.tb02096.x.
2
Immunochemistry on ultrathin frozen sections.超薄冰冻切片的免疫化学
Histochem J. 1980 Jul;12(4):381-403. doi: 10.1007/BF01011956.
3
A new 220,000 dalton protein located in the Z lines of vertebrate skeletal muscle.一种位于脊椎动物骨骼肌Z线的新型220,000道尔顿蛋白质。
J Biochem. 1981 Jun;89(6):1981-4. doi: 10.1093/oxfordjournals.jbchem.a133401.
4
The association of desmin with the developing myofibrils of cultured embryonic rat heart myocytes.结蛋白与培养的胚胎大鼠心肌细胞发育中的肌原纤维的关联。
Dev Biol. 1982 Jun;91(2):448-57. doi: 10.1016/0012-1606(82)90051-3.
5
The synthesis and distribution of desmin and vimentin during myogenesis in vitro.体外成肌过程中结蛋白和波形蛋白的合成与分布。
Cell. 1980 Jan;19(1):263-75. doi: 10.1016/0092-8674(80)90408-0.
6
Actin filament capping protein from bovine brain.来自牛脑的肌动蛋白丝封端蛋白。
EMBO J. 1982;1(7):889-94. doi: 10.1002/j.1460-2075.1982.tb01265.x.
7
Preparation of myofibrils.肌原纤维的制备
Methods Enzymol. 1982;85 Pt B:9-12. doi: 10.1016/0076-6879(82)85004-0.
8
Mechanism of action of Acanthamoeba profilin: demonstration of actin species specificity and regulation by micromolar concentrations of MgCl2.棘阿米巴肌动蛋白结合蛋白的作用机制：肌动蛋白种类特异性及微摩尔浓度氯化镁调控的证明
J Cell Biol. 1982 Jul;94(1):213-8. doi: 10.1083/jcb.94.1.213.
9
F actin assembly modulated by villin: Ca++-dependent nucleation and capping of the barbed end.由绒毛蛋白调节的F-肌动蛋白组装：钙离子依赖性的带刺末端成核和封端
Cell. 1981 May;24(2):471-80. doi: 10.1016/0092-8674(81)90338-x.
10
Reconstitution and regulation of actin gel-sol transformation with purified filamin and villin.利用纯化的细丝蛋白和绒毛蛋白对肌动蛋白凝胶-溶胶转变进行重构与调控。
J Biol Chem. 1981 Mar 10;256(5):2083-6.
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