从含亮绿的营养肉汤中分离出类志贺邻单胞菌：其在筛查非洲人群粪便样本中的应用
Isolation of Plesiomonas shigelloides from nutrient broth with brilliant green: its use in screening stool samples from an African population.
作者信息
Nair P, Millership S E
出版信息
J Clin Pathol. 1987 Jun;40(6):680-2. doi: 10.1136/jcp.40.6.680.
Abstract
The suitability and selectivity of nutrient broth with bile salts and varying concentrations of brilliant green as an enrichment medium was tested for the isolation of Plesiomonas shigelloides from faeces. The broth was most effective at a concentration of 0.001 g/l brilliant green, using faeces deliberately contaminated with Plesiomonas. Two hundred and nineteen faecal samples from Nigeria were then examined for the presence of Plesiomonas and other bacterial pathogens. One yielded Plesiomonas following enrichment in bile salts brilliant green broth (BBG broth) whereas no samples were positive on direct plating.
摘要
测试了含胆盐和不同浓度亮绿的营养肉汤作为增菌培养基用于从粪便中分离类志贺邻单胞菌的适用性和选择性。使用故意被类志贺邻单胞菌污染的粪便，该肉汤在亮绿浓度为0.001 g/l时效果最佳。随后对来自尼日利亚的219份粪便样本进行了检查，以检测类志贺邻单胞菌和其他细菌病原体的存在。一份样本在胆盐亮绿肉汤（BBG肉汤）增菌后检出类志贺邻单胞菌，而直接平板接种时没有样本呈阳性。
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