用于从人类粪便中分离气单胞菌属和邻单胞菌属的鉴别培养基和选择性培养基的评估。
Evaluation of differential and selective media for isolation of Aeromonas and Plesiomonas spp. from human feces.
作者信息
von Graevenitz A, Bucher C
出版信息
J Clin Microbiol. 1983 Jan;17(1):16-21. doi: 10.1128/jcm.17.1.16-21.1983.
Abstract
We studied nine solid and two liquid media for their suitability to select Aeromonas and Plesiomonas spp. from human stools, using artificially contaminated samples as well as 254 samples from outpatients with and without diarrhea. Media with optimal sensitivity and specificity for Aeromonas spp. were alkaline peptone-water, Trypticase soy broth with ampicillin, inositol-brilliant green-bile salts agar, dextrin-fuchsin-sulfite agar, xylose-sodium desoxycholate-citrate agar, and Pril-xylose-ampicillin agar. For Plesiomonas sp., alkaline peptone-water and inositol-brilliant green-bile salts agar were optimal. Four strains of Aeromonas spp. were detected in patient samples with these media.
摘要
我们研究了九种固体培养基和两种液体培养基，以评估它们从人类粪便中筛选气单胞菌属和邻单胞菌属细菌的适用性，使用人工污染样本以及254份来自有腹泻和无腹泻门诊患者的样本。对气单胞菌属具有最佳敏感性和特异性的培养基有碱性蛋白胨水、含氨苄青霉素的胰蛋白酶大豆肉汤、肌醇-煌绿-胆盐琼脂、糊精-品红-亚硫酸盐琼脂、木糖-脱氧胆酸钠-柠檬酸盐琼脂和普瑞-木糖-氨苄青霉素琼脂。对于邻单胞菌属，碱性蛋白胨水和肌醇-煌绿-胆盐琼脂是最佳的。使用这些培养基在患者样本中检测到了四株气单胞菌属细菌。
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Evaluation of differential and selective media for isolation of Aeromonas and Plesiomonas spp. from human feces.用于从人类粪便中分离气单胞菌属和邻单胞菌属的鉴别培养基和选择性培养基的评估。
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