Zhang Xueying, Liu Xinxin, Li Fadi, Yue Xiangpeng
State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.
Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin 733300, China.
Animals (Basel). 2020 Oct 1;10(10):1784. doi: 10.3390/ani10101784.
Colostrum and milk proteins are essential resources for the growth and development of the newborns, while their kinds and amounts vary greatly during the lactation period. This study was conducted to better understand whey proteome and its changes at six lactation time points (0 d, 3 d, 7 d, 14 d, 28 d, and 56 d after lambing) in Hu sheep. Using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF/TOF MS) technologies, a total of 52 differentially expressed protein spots (DEPS), corresponding to 25 differentially expressed proteins (DEPs), were obtained. The protein spots abundance analysis revealed that the proteins are the most abundant at 0 d after lambing. Gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to explore the biological functions of the DEPs. The biological process was mainly involved in localization, the single-organism process, the cellular process, and a series of immune processes. The cellular components engaged in the extracellular region were the cell, organelle, and membrane. The most prevalent molecular function was binding activity. In addition, the DEPs were involved in nine significant pathways, including the Hippo signaling pathway and Complement and coagulation cascades. These results intuitively presented the changes in Hu sheep whey proteins during a 56-d lactation period, and revealed potential biological functions of the DEPs, providing a scientific basis for early weaning.
初乳和乳蛋白是新生儿生长发育的重要资源，而它们的种类和含量在哺乳期会有很大差异。本研究旨在更好地了解湖羊在六个泌乳时间点（产羔后0天、3天、7天、14天、28天和56天）的乳清蛋白质组及其变化。利用二维凝胶电泳（2-DE）和基质辅助激光解吸/电离飞行时间质谱（MALDI TOF/TOF MS）技术，共获得了52个差异表达蛋白点（DEPS），对应25个差异表达蛋白（DEPs）。蛋白质点丰度分析表明，这些蛋白质在产羔后0天最为丰富。利用基因本体论（GO）注释和京都基因与基因组百科全书（KEGG）通路分析来探索差异表达蛋白的生物学功能。生物学过程主要涉及定位、单细胞过程、细胞过程以及一系列免疫过程。参与细胞外区域的细胞成分是细胞、细胞器和膜。最普遍的分子功能是结合活性。此外，差异表达蛋白涉及九条重要通路，包括Hippo信号通路和补体与凝血级联反应。这些结果直观地呈现了湖羊乳清蛋白在56天泌乳期内的变化，并揭示了差异表达蛋白的潜在生物学功能，为早期断奶提供了科学依据。
