Yang Mei, Cao Xueyan, Wu Rina, Liu Biao, Ye Wenhui, Yue Xiqing, Wu Junrui
a College of Food Science , Shenyang Agricultural University , Shenyang , PR China.
b Inner Mongolia Yili Industrial Group Company Limited , Hohhot , PR China.
Int J Food Sci Nutr. 2017 Sep;68(6):671-681. doi: 10.1080/09637486.2017.1279129. Epub 2017 Feb 20.
Whey, an essential source of dietary nutrients, is widely used in dairy foods for infants. A total of 584 whey proteins in human and bovine colostrum and mature milk were identified and quantified by the isobaric tag for relative and absolute quantification (iTRAQ) proteomic method. The 424 differentially expressed whey proteins were identified and analyzed according to gene ontology (GO) annotation, Kyoto encyclopedia of genes and genomes (KEGG) pathway, and multivariate statistical analysis. Biological processes principally involved biological regulation and response to stimulus. Major cellular components were extracellular region part and extracellular space. The most prevalent molecular function was protein binding. Twenty immune-related proteins and 13 proteins related to enzyme regulatory activity were differentially expressed in human and bovine milk. Differentially expressed whey proteins participated in many KEGG pathways, including major complement and coagulation cascades and in phagosomes. Whey proteins show obvious differences in expression in human and bovine colostrum and mature milk, with consequences for biological function. The results here increase our understanding of different whey proteomes, which could provide useful information for the development and manufacture of dairy products and nutrient food for infants. The advanced iTRAQ proteomic approach was used to analyze differentially expressed whey proteins in human and bovine colostrum and mature milk.
乳清是膳食营养的重要来源，广泛应用于婴儿乳制品中。采用相对和绝对定量等压标签（iTRAQ）蛋白质组学方法，对人初乳、牛初乳及成熟乳中的584种乳清蛋白进行了鉴定和定量。根据基因本体论（GO）注释、京都基因与基因组百科全书（KEGG）通路及多变量统计分析，对424种差异表达的乳清蛋白进行了鉴定和分析。生物学过程主要涉及生物调控和对刺激的反应。主要细胞成分是细胞外区域部分和细胞外空间。最普遍的分子功能是蛋白质结合。在人乳和牛乳中，20种免疫相关蛋白和13种与酶调节活性相关的蛋白差异表达。差异表达的乳清蛋白参与了许多KEGG通路，包括主要的补体和凝血级联反应以及吞噬体中的通路。人初乳、牛初乳及成熟乳中的乳清蛋白在表达上存在明显差异，这对生物学功能有影响。本文结果增进了我们对不同乳清蛋白质组的理解，可为婴儿乳制品和营养食品的开发与生产提供有用信息。采用先进的iTRAQ蛋白质组学方法分析人初乳、牛初乳及成熟乳中差异表达的乳清蛋白。
