Zaytsoff Sarah J M, Uwiera Richard R E, Inglis G Douglas
Agriculture and Agri-Food Canada, Lethbridge, AB, T1J 4B1, Canada.
Department of Agricultural, Food, and Nutritional Science, University of Alberta, 410 Agriculture/Forestry Centre, Edmonton, AB, T6G 2P5, Canada.
Microorganisms. 2020 Oct 1;8(10):1518. doi: 10.3390/microorganisms8101518.
A model of physiological stress mediated by the administration of corticosterone (CORT) was used to investigate the impact of stress on the intestinal microbiota of chickens. Birds were administered CORT in their drinking water at 0, 10 (low dose CORT; LDC), and 30 (high dose CORT; HDC) mg/L. Digesta from the small intestine and ceca were examined after 1, 5, and 12 days post-initiation of CORT administration by 16S rRNA gene sequencing. A decrease in phylogenetic diversity and altered composition of bacteria were observed for HDC in the small intestine. Analysis by ANOVA-Like Differential Expression 2 (ALDEx2) showed that densities of 1 bacteria were increased in the small intestine for LDC and HDC. Quantitative PCR confirmed that CORT administration increased densities of in the small intestine, but only HDC was associated with increased densities of the bacterium in ceca. Predictive functional analysis by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States 2 (PICRUSt2) showed pathways of carbohydrate metabolism to be enriched with CORT, and amino acid synthesis to be enriched in control birds in the small intestine. In conclusion, physiological stress mediated by CORT modulated bacterial communities in the small intestine and increased densities of . This implicates stress as an important mediator of this important enteric pathogen in poultry.
采用皮质酮（CORT）给药介导的生理应激模型，研究应激对鸡肠道微生物群的影响。给鸡饮用含0、10（低剂量CORT；LDC）和30（高剂量CORT；HDC）mg/L CORT的水。在开始给予CORT后的第1、5和12天，通过16S rRNA基因测序检查小肠和盲肠的食糜。观察到高剂量CORT组小肠的系统发育多样性降低，细菌组成改变。通过类方差分析差异表达2（ALDEx2）分析表明，低剂量和高剂量CORT组小肠中1种细菌的密度增加。定量PCR证实，给予CORT会增加小肠中的密度，但只有高剂量CORT组与盲肠中该细菌的密度增加有关。通过未观察状态重建的群落系统发育研究2（PICRUSt2）进行的预测功能分析表明，碳水化合物代谢途径在CORT组中富集，而氨基酸合成途径在小肠对照组中富集。总之，CORT介导的生理应激调节了小肠中的细菌群落并增加了的密度。这表明应激是家禽中这种重要肠道病原体的重要介导因素。
