Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
Faculty of Medicine, Alexandria University, Alexandria, Egypt.
J Biomater Appl. 2021 Jul;36(1):128-139. doi: 10.1177/0885328220963920. Epub 2020 Oct 5.
Over the past ten years, regenerative medicine has focused on the regeneration and the reconstruction of damaged, diseased, or lost tissues and organs. Skin, being the largest organ in the human body, had attained a good attraction in this field. Delayed wound healing is one of the most challenging clinical medicine complications. This study aimed to evaluate the collagen chitosan scaffold's effect alone, or enriched with either bone marrow-derived mesenchymal stem cells (BM-MSCs) or their secreted extracellular vesicles (EVs) on the duration and quality of skin wound healing.
A full-thickness skin wound was induced on the back of 32 adult male Sprague-Dawley rats. The wounds were either covered with collagen chitosan scaffolds alone, scaffolds enriched with stem cells, or extracellular vesicles. Unprotected wounds were used as control. Healing duration, collagen deposition and alignment, CD 68+ macrophage count, and functional tensile strength of healed skin were assessed (α = 0.05, n = 8).
The rate of skin healing was significantly accelerated in all treated groups compared to the control. Immuno-histochemical assessment of CD68+ macrophages showed enhanced macrophages count, in addition to higher collagen deposition and better collagen alignment in EVs and BM-MSCs treated groups compared to the control group. Higher tensile strength values reflected the better collagen deposition and alignment for these groups. EVs showed higher amounts of collagen deposition and better alignment compared to MSCs treated group.
The collagen chitosan scaffolds enriched with MSCs or their EVs improved wound healing and improved the quantity and remodeling of collagen with a better assignment to EVs.
在过去的十年中，再生医学一直专注于受损、患病或失去的组织和器官的再生和重建。皮肤作为人体最大的器官，在这一领域引起了广泛关注。延迟伤口愈合是最具挑战性的临床医学并发症之一。本研究旨在评估单独使用胶原壳聚糖支架，或用骨髓间充质干细胞（BM-MSCs）或其分泌的细胞外囊泡（EVs）丰富的胶原壳聚糖支架对皮肤伤口愈合的持续时间和质量的影响。
在 32 只成年雄性 Sprague-Dawley 大鼠的背部诱导全层皮肤伤口。伤口要么用单独的胶原壳聚糖支架覆盖，要么用富含干细胞的支架或细胞外囊泡覆盖。未受保护的伤口作为对照。评估愈合时间、胶原沉积和排列、CD68+巨噬细胞计数和愈合皮肤的功能拉伸强度（α=0.05，n=8）。
与对照组相比，所有治疗组的皮肤愈合速度均显著加快。CD68+巨噬细胞的免疫组织化学评估显示，与对照组相比，EVs 和 BM-MSCs 治疗组的巨噬细胞计数增加，胶原沉积增加，胶原排列更好。更高的拉伸强度值反映了这些组更好的胶原沉积和排列。EVs 组的胶原沉积量和排列情况均优于 MSC 治疗组。
用 MSC 或其 EVs 丰富的胶原壳聚糖支架可改善伤口愈合，并改善胶原的数量和重塑，且 EVs 组的胶原排列更好。
