Hadzieva N C, Hadziev S T
J Hyg Epidemiol Microbiol Immunol. 1987;31(2):189-95.
A method is developed for cultivating and observing bacteria in an early phase of their growth, when microcolonies were formed. The morphology of the microcolonies, including form, structure, characteristics of its periphery and center, as well as the mode of arrangement of bacteria in it and to each other proved to be typical for a species and often permitted its differentiation from the other species. Photographs are presented of typical microcolonies of S. aureus and of E. coli. A series of photographs is presented also as an illustration of the possibility to differentiate some species in the genus Streptococcus. The microcolonies observation is made 3 hours after material inoculation, that may permit a rapid bacteriological diagnosis. It is believed also, that the microcolony technique could be useful in the characterisation and identification of the species in the general bacteriology and taxonomy.
开发了一种用于在细菌生长早期培养和观察细菌的方法，此时会形成微菌落。微菌落的形态，包括形状、结构、周边和中心的特征，以及其中细菌彼此之间的排列方式，被证明是一个物种的典型特征，并且常常能够使其与其他物种区分开来。展示了金黄色葡萄球菌和大肠杆菌典型微菌落的照片。还展示了一系列照片，以说明区分链球菌属中某些物种的可能性。在接种材料3小时后进行微菌落观察，这可能有助于快速进行细菌学诊断。人们还认为，微菌落技术在普通细菌学和分类学中物种的表征和鉴定方面可能会很有用。
