Ozenci V, Tegmark-Wisell K, Lundberg C, Wretlind B
Division of Clinical Microbiology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden.
Clin Microbiol Infect. 2008 Feb;14(2):177-80. doi: 10.1111/j.1469-0691.2007.01897.x. Epub 2007 Dec 5.
This study describes the development of a method for rapid preliminary species identification of bacteria from positive blood culture vials. The method yielded preliminary identification results for 496 (92%) of 541 positive blood cultures within 5 h. The method was capable of identifying the most frequently isolated bacteria (i.e., Staphylococcus aureus, coagulase-negative staphylococci, Escherichia coli, Streptococcus pneumoniae and Enterococcus spp.) to the species level. The method can be established easily, with a materials cost of 2-5 Euros per sample.
本研究描述了一种用于从阳性血培养瓶中快速初步鉴定细菌种类的方法的开发。该方法在5小时内对541份阳性血培养中的496份（92%）产生了初步鉴定结果。该方法能够将最常分离出的细菌（即金黄色葡萄球菌、凝固酶阴性葡萄球菌、大肠杆菌、肺炎链球菌和肠球菌属）鉴定到种水平。该方法易于建立，每份样品的材料成本为2至5欧元。
