Lexer G, Cooper D K, Rose A G, Wicomb W N, Rees J, Keraan M, Du Toit E
J Heart Transplant. 1986 Nov-Dec;5(6):411-8.
Eight freshly excised pig hearts were hemoperfused by baboons in vivo. Cardiac function ceased in six hearts after a mean period of 90 minutes of perfusion (range 30 to 190 minutes). Two hearts continued functioning for the 4-hour study period. On microscopic examination, seven hearts, including one that continued beating, showed histopathologic features of hyperacute rejection. IgG, IgM, and C3 were strongly present on the myocardium in all cases. Hemoperfusion was associated with significant increases in heart mass (p less than 0.002) and circulating polymorphonuclear leukocytes (p less than 0.03) and reductions in coronary blood flow (p less than 0.0001), circulating lymphocytes (p less than 0.03), and C4 (p less than 0.03). Preformed circulating anti-pig antibodies disappeared in all but one baboon. Hyperacute rejection in this discordant xenograft model that used a nonhuman primate as "recipient" differs in few aspects from that seen in other experimental models. Possible methods of prolonging xenograft survival are discussed.
八颗刚切除的猪心脏在体内由狒狒进行血液灌注。平均灌注90分钟（范围为30至190分钟）后，六颗心脏的心脏功能停止。两颗心脏在4小时的研究期内持续发挥功能。显微镜检查显示，七颗心脏，包括一颗仍在跳动的心脏，呈现超急性排斥反应的组织病理学特征。所有病例的心肌上均强烈存在IgG、IgM和C3。血液灌注与心脏重量显著增加（p<0.002）、循环多形核白细胞增加（p<0.03）以及冠状动脉血流量减少（p<0.0001）、循环淋巴细胞减少（p<0.03）和C4减少（p<0.03）相关。除一只狒狒外，所有预先形成的循环抗猪抗体均消失。在这种使用非人类灵长类动物作为“受体”的异种移植不匹配模型中，超急性排斥反应在几个方面与其他实验模型中所见的情况不同。文中讨论了延长异种移植存活时间的可能方法。
