Krüger D H, Bickle T A
J Mol Biol. 1987 Mar 20;194(2):349-52. doi: 10.1016/0022-2836(87)90382-2.
The use of different precisely mapped T3/T7 recombinants strengthens the conclusion that abortive infection by T7 of F plasmid-carrying cells is due to the nucleotide sequence at the end of the T7 gene 1. Furthermore, we demonstrate that the exclusion requires suppression of ochre stop codons, a phenomenon that occurs with low frequency in wild-type cells due to ribosomal misreading. The introduction of rspL mutations in which ribosomal misreading is reduced alleviates the exclusion and the presence of ochre tRNA suppressors increases its severity.
使用不同的精确映射的T3/T7重组体强化了这样的结论，即携带F质粒的细胞被T7进行流产感染是由于T7基因1末端的核苷酸序列。此外，我们证明这种排斥需要抑制赭石色终止密码子，由于核糖体错读，这种现象在野生型细胞中发生频率较低。引入降低核糖体错读的rspL突变可减轻这种排斥，而赭石色tRNA抑制子的存在会增加其严重程度。
