Beck P J, Molineux I J
Department of Microbiology, University of Texas, Austin 78712.
J Bacteriol. 1991 Feb;173(3):947-54. doi: 10.1128/jb.173.3.947-954.1991.
Transcription of T7 and mutant T3 DNA during infections of F plasmid-containing cells has been analyzed by using Southern hybridization. A transcriptional defect is apparent in these abortively infected cells that is most severe in the class III region of the phage genome. In particular, RNAs that are initiated from the gene 13 promoter are not elongated to give full-length molecules. It is suggested that the transcription defect results from positive supercoiling of the template DNA and that torsional constraints may even prevent the complete entry of the phage genome into an abortively infected cell.
通过Southern杂交分析了含F质粒细胞感染期间T7和突变T3 DNA的转录情况。在这些流产感染的细胞中明显存在转录缺陷，在噬菌体基因组的III类区域最为严重。特别是，从基因13启动子起始的RNA不会延伸形成全长分子。有人认为转录缺陷是由模板DNA的正超螺旋导致的，并且扭转限制甚至可能阻止噬菌体基因组完全进入流产感染的细胞。
