Laboratory Animal Resource Center, School of Medicine, Indiana University, Indianapolis, Indiana;, Email:
J Am Assoc Lab Anim Sci. 2021 Jan 1;60(1):72-76. doi: 10.30802/AALAS-JAALAS-20-000055. Epub 2020 Oct 6.
The AVMA Guidelines on Euthanasia state that, to decrease potential distress of animals, the home cage should be used for the euthanasia of mice. The current study evaluated this recommendation by comparing behavioral and physiologic changes in ICR and SJL mice that were euthanized by using a 30% volume per minute displacement rate of 100% CO₂ in either their home cage or an induction chamber. Blood samples were collected to assess blood glucose, serum corticosterone, and serum noradrenaline as markers of physiologic wellbeing. Behavioral assessment was performed (with emphasis on behaviors including rearing, jumping, sniffing at the gas inlet, and grooming) from the introduction of gas to the estimated time to loss of consciousness (i. e., the time period when the animal would be expected to experience pain or distress). Despite significant differences between mouse strains, no significant differences were detected in the physiologic or behavioral parameters assessed when comparing the home cage with the induction chamber. This finding suggests that- from the perspective of a mouse-either the home cage or an induction chamber can be used for induction of anesthesia with CO₂ during the euthanasia procedure.
美国兽医协会（AVMA）的安乐死指南规定，为减少动物的潜在痛苦，应在家用笼子中对老鼠实施安乐死。本研究通过比较在其家用笼子或诱导室中，以 30%每分钟体积的 100%二氧化碳置换率对 ICR 和 SJL 小鼠实施安乐死时，其行为和生理变化，评估了这一建议。采集血样以评估血糖、血清皮质酮和血清去甲肾上腺素作为生理健康的标志物。从引入气体到估计失去意识的时间（即动物预计会经历疼痛或痛苦的时间段）进行行为评估（重点评估包括饲养、跳跃、嗅探气体入口和梳理等行为）。尽管在两种小鼠品系之间存在显著差异，但在比较家用笼子和诱导室时，评估的生理或行为参数没有发现显著差异。这一发现表明，从老鼠的角度来看，在安乐死过程中，家用笼子或诱导室都可以用于用二氧化碳诱导麻醉。
