志贺毒素A和B亚基的物理化学特性以及从分离的亚基重构全毒素
Physicochemical characterization of A and B subunits of Shiga toxin and reconstitution of holotoxin from isolated subunits.
作者信息
Yutsudo T, Honda T, Miwatani T, Takeda Y
出版信息
Microbiol Immunol. 1987;31(3):189-97. doi: 10.1111/j.1348-0421.1987.tb03083.x.
The A and B subunits of Shiga toxin were isolated by high performance liquid chromatography and their physicochemical properties were examined. The A subunit of Shiga toxin purified from culture supernatant was not nicked, but it could be nicked in vitro by trypsin. The isoelectric points of the A and B subunits were determined to be 8.2 and 5.8, respectively. Amino acid compositions of the two subunits were also determined. The isolated A and B subunits were reconstituted to form active holotoxin which showed lethal activity to mice which was similar to that of native Shiga toxin.
通过高效液相色谱法分离出志贺毒素的A亚基和B亚基，并对其理化性质进行了检测。从培养上清液中纯化得到的志贺毒素A亚基未被切割，但在体外可被胰蛋白酶切割。测定A亚基和B亚基的等电点分别为8.2和5.8。还测定了两个亚基的氨基酸组成。将分离得到的A亚基和B亚基重新组装形成有活性的全毒素，该全毒素对小鼠表现出与天然志贺毒素相似的致死活性。
Zotero 插件