痢疾志贺氏菌产生的一种致死毒素的纯化
Purification of a lethal toxin produced by Shigella dysenteriae.
作者信息
Okamoto K, Takeda Y, Miwatani T
出版信息
Toxicon. 1982;20(2):451-6. doi: 10.1016/0041-0101(82)90008-3.
A lethal toxin was purified from the culture supernatant of Shigella dysenteriae 1. The purification procedure utilized ammonium sulfate fractionation, column chromatography on DEAE-cellulose, CM-cellulose, hydroxylapatite and gel filtration on Sephadex G-200. About a 4760-fold purification was achieved, with a yield of 2.7%. The purified lethal toxin appeared homogeneous on polyacrylamide gel disc electrophoresis. The LD50 of the purified lethal toxin in mice following i.p. injection was about 7.5 micrograms/kg. Injection i.p. of 2 LD50 of the purified lethal toxin into mice caused paralysis of the limbs followed by death after 2-4 days.
从痢疾志贺氏菌1型的培养上清液中纯化出一种致死毒素。纯化步骤包括硫酸铵分级分离、DEAE-纤维素柱色谱、CM-纤维素柱色谱、羟基磷灰石柱色谱以及Sephadex G-200凝胶过滤。实现了约4760倍的纯化，产率为2.7%。纯化后的致死毒素在聚丙烯酰胺凝胶圆盘电泳上呈现均一性。纯化后的致死毒素经腹腔注射对小鼠的半数致死量约为7.5微克/千克。给小鼠腹腔注射2倍半数致死量的纯化致死毒素会导致四肢麻痹，2至4天后死亡。
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