Vorob'ev M M, Vitt S V, Belikov V M
Nahrung. 1987;31(4):331-40. doi: 10.1002/food.19870310422.
A theoretical analysis of the general kinetics of peptide bond hydrolysis in peptide mixtures has been carried out. It is shown that the equation for the hydrolysis rate must include ensemble-averaged values which change as functions during hydrolysis. Thus the problem of describing the hydrolysis is reduced to finding these functions. As an example of theoretical prediction for the behavior of the averaged functions the procedure of calculating the averaged constant of chymotrypsin substrate specificity as a function of the degree of hydrolysis is presented. The analysis performed enables to find factors responsible for the decrease of hydrolysis rate, in particular, the role of the substrate specificity range and the S/E ratio. The design of specific experiments making it possible to measure the averaged values in the course of hydrolysis has been considered.
对肽混合物中肽键水解的一般动力学进行了理论分析。结果表明，水解速率方程必须包含在水解过程中作为函数变化的系综平均值。因此，描述水解的问题就归结为找到这些函数。作为对平均函数行为的理论预测示例，给出了计算作为水解度函数的胰凝乳蛋白酶底物特异性平均常数的过程。所进行的分析能够找出导致水解速率降低的因素，特别是底物特异性范围和S/E比的作用。还考虑了设计特定实验以便能够在水解过程中测量平均值。
