Chong G L, Davis L C, Reeck G R
Department of Biochemistry, Kansas State University, Manhattan 66506.
Anal Biochem. 1990 May 1;186(2):269-72. doi: 10.1016/0003-2697(90)90078-n.
A flow-injection analysis (FIA) system was developed to study the enzyme-catalyzed hydrolysis of synthetic peptides, each of which contained one scissile bond. The concentrations of alpha-amino groups in reactions mixtures were determined by FIA with o-phthalaldehyde as a fluorescence reagent. The method allows a rapid, precise, and sensitive determination of kinetic constants for proteases acting on extended peptide substrates.
