Biology Centre, Czech Academy of Sciences, Institute of Parasitology, Branišovská 31, 37005, České Budějovice, Czech Republic.
Department of Molecular Biology, Faculty of Science, University of South Bohemia, Branišovská 31, 37005, České Budějovice, Czech Republic.
Sci Rep. 2020 Oct 7;10(1):16687. doi: 10.1038/s41598-020-73715-8.
Eukaryotic complex phototrophs exhibit a colorful evolutionary history. At least three independent endosymbiotic events accompanied by the gene transfer from the endosymbiont to host assembled a complex genomic mosaic. Resulting patchwork may give rise to unique metabolic capabilities; on the other hand, it can also blur the reconstruction of phylogenetic relationships. The ornithine-urea cycle (OUC) belongs to the cornerstone of the metabolism of metazoans and, as found recently, also photosynthetic stramenopiles. We have analyzed the distribution and phylogenetic positions of genes encoding enzymes of the urea synthesis pathway in eukaryotes. We show here that metazoan and stramenopile OUC enzymes share common origins and that enzymes of the OUC found in primary algae (including plants) display different origins. The impact of this fact on the evolution of stramenopiles is discussed here.
真核复杂光合生物表现出丰富多彩的进化历史。至少有三个独立的内共生事件伴随着从内共生体到宿主的基因转移，组装成一个复杂的基因组镶嵌体。由此产生的拼凑可能会产生独特的代谢能力；另一方面，它也可能模糊系统发育关系的重建。精氨酸-尿素循环（OUC）是后生动物代谢的基石，最近也在光合不等鞭毛类中发现。我们分析了编码尿素合成途径酶的基因在真核生物中的分布和系统发育位置。我们在这里表明，后生动物和不等鞭毛类的 OUC 酶具有共同的起源，而在初级藻类（包括植物）中发现的 OUC 酶具有不同的起源。本文讨论了这一事实对不等鞭毛类进化的影响。
