Departamento de Ingeniería Celular Y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México. Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos, 62210, México.
Facultad de Ciencias Quıímico Biológicas, Universidad Autónoma de Guerrero, Av. Lazaro Cardenas S/N. Cd. Universitaria, 39070, Chilpancingo, Guerrero, Mexico.
Bioprocess Biosyst Eng. 2021 Feb;44(2):379-389. doi: 10.1007/s00449-020-02450-1. Epub 2020 Oct 7.
The effectiveness of micro-aeration on lactate (LA) production by metabolically engineered Escherichia coli was evaluated in 1 L bioreactors containing mineral media and glucose (70 g/L). Volumetric oxygen transfer coefficients (ka) between 12.6 and 28.7 h increased the specific growth rate (µ) and volumetric productivity (Q) by 300 and 400%, respectively, without a significant decrease in lactate yield (Y), when compared with non-aerated fermentations. A ka of 12.6 h was successfully used as a criterion to scale-up the production of L and D-lactate from 1 to 11 and 130 L. Approximately constant Q and Y values were obtained throughout the fermentation scale-up process. Furthermore, a D-lactogenic fermentation was carried out in 1 L bioreactors using avocado seed hydrolysate as a culture medium under the same ka value, displaying high Q and Y.
在含有矿物培养基和葡萄糖（70g/L）的 1L 生物反应器中，评估了微曝气对代谢工程大肠杆菌生产乳酸（LA）的效果。与非曝气发酵相比，体积氧传递系数（ka）在 12.6 到 28.7h 之间分别将比生长速率（µ）和比体积产率（Q）提高了 300%和 400%，而乳酸产率（Y）没有显著下降。12.6h 的 ka 成功地用作从 1L 放大到 11L 和 130L 的 L 和 D-乳酸生产的标准。在整个发酵放大过程中，均获得了大致恒定的 Q 和 Y 值。此外，在相同的 ka 值下，使用鳄梨籽水解物作为培养基，在 1L 生物反应器中进行了 D-乳酸发酵，显示出了高的 Q 和 Y。
