一种用于构建基因文库以及在酿酒酵母中表达异源基因的载体。

A vector for construction of gene libraries and the expression of heterologous genes in Saccharomyces cerevisiae.

作者信息

Khan M I, Ecker D J, Butt T, Gorman J A, Crooke S T

出版信息

Plasmid. 1987 Mar;17(2):171-2. doi: 10.1016/0147-619x(87)90024-2.

Abstract

We have constructed a convenient new vector, YEp-DE, for the construction of gene libraries and the expression of heterologous genes in Saccharomyces cerevisiae. The vector contains the yeast LEU2 gene, the 2 mu origin of replication, and a region from pUC18 that includes the ampr gene, the Escherichia coli origin of replication (ori), and the LacZ gene with multiple cloning sites. Five sites (Sac1, Sma1, BamH1, Sal1, Sph1) in this region are unique. This vector has advantages over similar yeast-E. coli shuttle vectors: small size (7291 bp, entirely sequenced), convenient cloning sites, and lacZ selection for detecting recombinant plasmids.

摘要

我们构建了一种便捷的新型载体YEp-DE，用于构建基因文库以及在酿酒酵母中表达异源基因。该载体包含酵母LEU2基因、2μm复制起点，以及来自pUC18的一个区域，该区域包括氨苄青霉素抗性基因（ampr）、大肠杆菌复制起点（ori）和带有多个克隆位点的LacZ基因。此区域的五个位点（Sac1、Sma1、BamH1、Sal1、Sph1）是独一无二的。与类似的酵母-大肠杆菌穿梭载体相比，该载体具有以下优点：体积小（7291 bp，全序列已测定）、克隆位点便捷，以及可通过LacZ选择来检测重组质粒。