Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan 430070, P.R. China.
College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, P.R. China.
J Agric Food Chem. 2020 Oct 21;68(42):11863-11874. doi: 10.1021/acs.jafc.0c05153. Epub 2020 Oct 8.
Fruit and leaf possess distinctly different metabolites. Here, metabolites and transcriptome were compared between mature leaves (ML) and juice sacs (JS) of "Hirado Buntan" to investigate the possible reasons. Results indicated that the remarkable difference in starch, total flavonoids and carotenoids, l-ascorbate, and jasmonic acid between ML and JS was tightly related to the expression levels of their biosynthesis-related genes, while the significant difference in abscisic acid and citrate was mainly related to the gene expression level(s) of 9--epoxycarotenoid dioxygenase and proton pump genes, respectively. In addition, ATP citrate lyase probably plays a key role in the levels of flavonoids between ML and JS via providing different levels of acetyl-CoA. Taken together, these results identified some key candidate genes responsible for the content of a given metabolite and will contribute to research in regulating such metabolite content in citrus fruits.
果实和叶片具有明显不同的代谢物。在这里，通过比较“平户文旦”成熟叶片（ML）和汁囊（JS）之间的代谢物和转录组，来探究可能的原因。结果表明，ML 和 JS 之间淀粉、总类黄酮和类胡萝卜素、l-抗坏血酸和茉莉酸的显著差异与它们生物合成相关基因的表达水平密切相关，而脱落酸和柠檬酸的显著差异主要与 9--环氧类胡萝卜素双加氧酶和质子泵基因的基因表达水平相关。此外，ATP 柠檬酸裂解酶可能通过提供不同水平的乙酰辅酶 A 在 ML 和 JS 之间的类黄酮水平中发挥关键作用。总的来说，这些结果确定了一些负责特定代谢物含量的关键候选基因，这将有助于柑橘果实中此类代谢物含量的调控研究。
