Key Laboratory of Horticultural Plant Biology (Ministry of Education) and Key Laboratory of Horticultural Crop Biology and Genetic Improvement, Central Region (Ministry of Agriculture), Huazhong Agricultural University , Wuhan 430070, PR China.
Quzhou Bureau of Agriculture Economic Specialty Station , Quzhou 324000, Zhejiang Province, PR China.
J Agric Food Chem. 2015 Sep 30;63(38):8601-14. doi: 10.1021/acs.jafc.5b02807. Epub 2015 Sep 21.
As the most important fresh fruit worldwide, citrus is often subjected to huge postharvest losses caused by abiotic and biotic stresses. As a promising strategy to reduce postharvest losses, enhancing natural defense by potential metabolism reprogramming in citrus mutants has rarely been reported. The yellowish spontaneous mutant of Ponkan (Citrus reticulata Blanco) (YP) was used to investigate the influence of metabolism reprogramming on postharvest performance. Our results show that reduced xanthophyll accumulation is the cause of yellowish coloring of YP and might be attributed to the reduced carotenoid sequestration capacity and upregulated expression of carotenoid cleavage dioxygenase genes. Constantly higher levels of polymethoxylated flavones (PMFs) during the infection and the storage stage might make significant contribution to the more strongly induced resistance against Penicillium digitatum and lower rotting rate. The present study demonstrates the feasibility of applying bud mutants to improve the postharvest performance of citrus fruits.
作为全球最重要的新鲜水果之一，柑橘常因非生物和生物胁迫而遭受巨大的产后损失。通过柑橘突变体的潜在代谢重编程来增强天然防御能力，是一种减少产后损失的有前景的策略，但鲜有报道。本研究利用甜橙（Citrus reticulata Blanco）的自然黄化突变体（YP）来研究代谢重编程对产后性能的影响。研究结果表明，类胡萝卜素积累减少是 YP 果实黄化的原因，这可能归因于类胡萝卜素的积累能力降低和类胡萝卜素双加氧酶基因的表达上调。在感染和贮藏阶段持续高水平的多甲氧基黄酮（PMFs）可能对诱导对绿霉更强的抗性和降低腐烂率有重要贡献。本研究表明，芽变突变体在改善柑橘果实产后性能方面具有可行性。
