Piruzian E S, Kobets N S, Alikhanian S I
Genetika. 1977;13(4):681-8.
Thin map of gene 43, controlling the synthesis of T4 DNA polymerase, is obtained by mapping experiments performed with 39 amber mutants, and is used for analysis of the sites of DNA polymerase gene from the point of view of displaying the mutator effect. The mutant sites studied possessed different reaction on amino acid substitutions in the polypeptide chain of the enzyme. Most of sites of the DNA polymerase gene, with the exception of two "supersensitive", responsed only on the apparent type of the amino acid substitutions: the mutator effect of amber mutations, which are located at these sites, was exhibited only in the case of insertion of the definite amino acid in the respective point of polypeptide chain. The proposed system of amber mutations for studying the mutator effect, allowed the authors to obtain the data on the effect of concrete alterations in the polypeptide chain of the enzyme on the development of its mutator properties.
通过对39个琥珀突变体进行定位实验，获得了控制T4 DNA聚合酶合成的基因43的精细图谱，并从显示诱变效应的角度用于分析DNA聚合酶基因的位点。所研究的突变位点在酶的多肽链中对氨基酸取代有不同反应。DNA聚合酶基因的大多数位点，除了两个“超敏感”位点外，仅对氨基酸取代的表观类型有反应：位于这些位点的琥珀突变的诱变效应仅在多肽链相应位点插入特定氨基酸时才表现出来。所提出的用于研究诱变效应的琥珀突变系统，使作者能够获得关于酶多肽链中具体改变对其诱变特性发展影响的数据。
