Laboratory for Reproductive Endocrinology and Signaling, Laboratory for Chronobiology and Aging, Center of Excellence for Reproductive Endocrinology (CeRES), DBE, Faculty of Sciences, University of Novi Sad, Dositeja Obradovica Square 2, 21000, Novi Sad, Serbia.
Sci Rep. 2020 Oct 8;10(1):16813. doi: 10.1038/s41598-020-73630-y.
Here we investigate the stress-signaling responsible for the effects of acute/repeated psychological stresses (the most common stresses in human society) on spermatozoa number and functionality, as well as the transcriptional profile of mitochondrial dynamics markers by using the in vivo and ex vivo approaches. Acute and repeated stress inhibit spermatozoa functionality (acute -> 3.2-fold, repeated -> 2.5-fold), while only repeated stress reduces the spermatozoa number (1.7-fold). Stress hormones mimic these effects and decrease the spermatozoa functionality (adrenaline: 10 µM -> 2.4-fold, 100 µM - > 2.8-fold; hydrocortisone: 50 pM -> 2.7-fold, 500 pM -> 8.5-fold). They also significantly disturb the transcriptional profile of all main mitochondrial dynamics markers in spermatozoa. Ex vivo manipulation of stress signaling in spermatozoa reveals that most of these effects are mediated through ɑ1-and/or-β-adrenergic receptors. The transcription of these receptors and their kinases in the same samples is under the significant influence of adrenergic signaling. Our results are the first to show the importance of mitochondrial dynamics markers in spermatozoa since the transcriptional profiles of sixteen-out-of-ninteen are disturbed by manipulation of stress-hormones-signaling. This is a completely new molecular approach to assess spermatozoa functionality and it is important for a better understanding of the correlations between stress, environmental-life-style and other factors, and male (in)fertility.
在这里，我们通过体内和体外方法研究了负责急性/重复心理应激（人类社会中最常见的应激）对精子数量和功能的影响的应激信号，以及线粒体动力学标志物的转录谱。急性和重复应激抑制精子功能（急性 -> 3.2 倍，重复 -> 2.5 倍），而只有重复应激会减少精子数量（1.7 倍）。应激激素模拟这些效应并降低精子功能（肾上腺素：10 μM -> 2.4 倍，100 μM -> 2.8 倍；氢化可的松：50 pM -> 2.7 倍，500 pM -> 8.5 倍）。它们还显着扰乱了精子中线粒体动力学主要标志物的转录谱。精子中应激信号的体外操作表明，这些效应中的大多数是通过α1-和/或β-肾上腺素受体介导的。这些受体及其在同一样本中的激酶的转录受肾上腺素信号的显着影响。我们的研究结果首次表明，线粒体动力学标志物在精子中的重要性，因为十六分之十九的转录谱因应激激素信号转导的操纵而受到干扰。这是一种评估精子功能的全新分子方法，对于更好地理解应激、环境-生活方式和其他因素与男性（不孕）不育之间的相关性非常重要。
