Ramirez Gaëlle, Gomez Elena, Dumas Thibaut, Rosain David, Mathieu Olivier, Fenet Hélène, Courant Frédérique
HydroSciences Montpellier, University of Montpellier, CNRS, IRD, Montpellier, France.
Laboratoire de Pharmacologie-Toxicologie, CHU de Montpellier, Montpellier, France.
Metabolites. 2022 Feb 22;12(3):197. doi: 10.3390/metabo12030197.
There is growing evidence of the presence of pharmaceuticals in natural waters and their accumulation in aquatic organisms. While their mode of action on non-target organisms is still not clearly understood, their effects warrant assessment. The present study assessed the metabolome of the Mediterranean mussel () exposed to a 10 µg/L nominal concentration of the antidepressant venlafaxine (VLF) at 3 time-points (1, 3, and 7 days). Over the exposure period, we observed up- or down-modulations of 113 metabolites, belonging to several metabolisms, e.g., amino acids (phenylalanine, tyrosine, tryptophan, etc.), purine and pyrimidine metabolisms (adenosine, cyclic AMP, thymidine, etc.), and several other metabolites involved in diverse functions. Serotonin showed the same time-course modulation pattern in both male and female mussels, which was consistent with its mode of action in humans, i.e., after a slight decrease on the first day of exposure, its levels increased at day 7 in exposed mussels. We found that the modulation pattern of impacted metabolites was not constant over time and it was gender-specific, as male and female mussels responded differently to VLF exposure.
越来越多的证据表明天然水体中存在药物及其在水生生物体内的积累。虽然它们对非目标生物的作用模式仍未完全清楚，但它们的影响值得评估。本研究评估了地中海贻贝在3个时间点（1天、3天和7天）暴露于标称浓度为10 µg/L的抗抑郁药文拉法辛（VLF）后的代谢组。在暴露期间，我们观察到113种代谢物的上调或下调，这些代谢物属于多种代谢途径，例如氨基酸（苯丙氨酸、酪氨酸、色氨酸等）、嘌呤和嘧啶代谢（腺苷、环磷酸腺苷、胸苷等）以及其他一些参与多种功能的代谢物。血清素在雄性和雌性贻贝中表现出相同的时间进程调节模式，这与其在人体内的作用模式一致，即在暴露第一天略有下降后，暴露贻贝在第7天其水平升高。我们发现，受影响代谢物的调节模式随时间并不恒定，且具有性别特异性，因为雄性和雌性贻贝对VLF暴露的反应不同。
