Poonsri Warinthon
Department of Agricultural Products Processing Engineering, Faculty of Agricultural Technology, Rajamangala University of Technology Thanyaburi, Pathumthani 12110, Thailand.
Heliyon. 2020 Sep 28;6(9):e05070. doi: 10.1016/j.heliyon.2020.e05070. eCollection 2020 Sep.
This research investigated the effect of different atmosphere storage conditions on 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase enzyme activity; and senescence of cut orchid flowers. The atmosphere storage conditions under study were normal atmosphere, modified atmosphere packaging, and controlled atmosphere. Under the normal atmosphere, carbon dioxide (CO) and oxygen (O) concentrations were 0.03 and 21 %, respectively. For the modified atmosphere packaging, cut orchid flowers were wrapped in polypropylene film prior to filling with 5 % CO and 2 % O, while under the controlled atmosphere, CO and O concentrations were maintained at 5 and 2 %, respectively. The storage temperature and relative humidity were 13 °C and 95 %, respectively. The ACC synthase and ACC oxidase activity and ethylene-induced electrolyte leakage were determined and results compared. The controlled atmosphere substantially lowered ACC synthase and ACC oxidase activity and was effective in delaying senescence of cut orchid flowers, as indicated by the longest storage life of 28.33 days, followed by the modified atmosphere packaging (18.15 days) and normal atmosphere (11.67 days). The longer storage life enables suppliers of orchid flowers to efficiently manage the demand and supply and also provides exporters with new opportunities to expand into distant overseas markets. The novelty of this research lies in the use of different storage environments to investigate the senescence mechanisms at tissue level of orchid flowers in response to ACC synthase and ACC oxidase enzyme activity.
本研究调查了不同气调贮藏条件对1-氨基环丙烷-1-羧酸（ACC）合成酶和ACC氧化酶活性以及切花兰花衰老的影响。所研究的气调贮藏条件包括正常大气、气调包装和控制气氛。在正常大气条件下，二氧化碳（CO）和氧气（O）浓度分别为0.03%和21%。对于气调包装，切花兰花先用聚丙烯薄膜包裹，然后充入5%的CO和2%的O，而在控制气氛条件下，CO和O浓度分别保持在5%和2%。贮藏温度和相对湿度分别为13℃和95%。测定了ACC合成酶和ACC氧化酶活性以及乙烯诱导的电解质渗漏，并对结果进行了比较。控制气氛显著降低了ACC合成酶和ACC氧化酶的活性，并有效地延缓了切花兰花的衰老，其贮藏寿命最长，为28.33天，其次是气调包装（18.15天）和正常大气（11.67天）。较长的贮藏寿命使兰花供应商能够有效地管理供需，也为出口商提供了拓展到遥远海外市场的新机会。本研究的新颖之处在于利用不同的贮藏环境，从组织水平研究兰花响应ACC合成酶和ACC氧化酶活性的衰老机制。
