Fisher-Smikle M, James O B
Trans R Soc Trop Med Hyg. 1987;81(2):255-6. doi: 10.1016/0035-9203(87)90232-x.
The Crithidia luciliae indirect immunofluorescence test (CL-IFT) was compared with the DNA-binding assay for confirmation of the presence of double-stranded DNA (dsDNA) antibodies in cases of systemic lupus erythematosus (SLE). The study involved 142 patients whose sera had anti-nuclear antibodies (ANA). In 62 patients with clinical or suspected SLE, 66% gave positive CL-IFT results as against 69% by DNA-binding. Two patients who had negative CL-IFT results but positive DNA-binding were only marginally positive (31% and 36%). Anti-dsDNA antibody was not found in any ANA negative sera. C. luciliae is easily maintained in culture and the test is relatively easy to perform in any laboratory with facilities for doing fluorescence microscopy; we recommend it for confirmation of the diagnosis of SLE, especially in laboratories with limited resources.
将利什曼原虫间接免疫荧光试验（CL-IFT）与DNA结合试验进行比较，以确认系统性红斑狼疮（SLE）患者血清中双链DNA（dsDNA）抗体的存在情况。该研究纳入了142例血清中有抗核抗体（ANA）的患者。在62例临床诊断或疑似SLE的患者中，CL-IFT结果阳性的占66%，而DNA结合试验结果阳性的占69%。两名CL-IFT结果阴性但DNA结合试验结果阳性的患者仅呈弱阳性（分别为31%和36%）。在ANA阴性的血清中未发现抗dsDNA抗体。利什曼原虫易于在培养中维持，并且该试验在任何具备荧光显微镜设备的实验室中相对容易操作；我们推荐使用该试验来确诊SLE，尤其是在资源有限的实验室。
