将抗双链DNA作为诊断工具进行检测。
Detection of anti-dsDNA as diagnostic tool.
作者信息
Swaak A J, Groenwold J, Aarden L A, Feltkamp T E
出版信息
Ann Rheum Dis. 1981 Feb;40(1):45-9. doi: 10.1136/ard.40.1.45.
Abstract
The diagnostic significance of anti-dsDNA determinations was evaluated in 2 different groups of patients. When the immunofluorescence technique (IFT) with Crithidia luciliae and the Farr assay with 3H-labelled-PM2 DNA were applied to a selected panel of 536 sera from patients with various well-defined autoimmune diseases, positive results were obtained only with serum samples from patients with systemic lupus erythematosus (SLE). On the other hand when we screened 4431 sera sent to our laboratory for diagnostic reasons, we observed a high incidence of antibodies to dsDNA in patients who did not fulfil the preliminary American Rheumatism Association's criteria for SLE and did not have the diagnosis SLE. Furthermore, a significant number of the positive sera showed peculiar behaviour in that they were positive only in the IFT on Crithidia luciliae and not in the Farr assay.
摘要
在两组不同的患者中评估了抗双链DNA检测的诊断意义。当将利什曼原虫免疫荧光技术（IFT）和用3H标记的PM2 DNA进行的Farr检测应用于从患有各种明确自身免疫性疾病的患者中选取的536份血清样本时，仅在系统性红斑狼疮（SLE）患者的血清样本中获得了阳性结果。另一方面，当我们筛查因诊断原因送到我们实验室的4431份血清时，我们发现在未达到美国风湿病协会SLE初步标准且未被诊断为SLE的患者中，抗双链DNA抗体的发生率很高。此外，相当数量的阳性血清表现出特殊行为，即它们仅在利什曼原虫IFT中呈阳性，而在Farr检测中呈阴性。
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