University of Silesia in Katowice, Department of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, Bankowa 9, PL 40-007, Katowice, Poland.
University of Silesia in Katowice, Department of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, Bankowa 9, PL 40-007, Katowice, Poland.
Environ Pollut. 2021 Jan 1;268(Pt A):115366. doi: 10.1016/j.envpol.2020.115366. Epub 2020 Aug 23.
Cadmium as a common environmental stressor may exert highly toxic effects on herbivorous insects. The question was whether possible elevation of an oxidative stress and imbalance of energetic reserves in insects may depend on developmental stage, sex and insect population's multigenerational history of exposure to cadmium. So, the aim of this study was to compare of the development traits, total antioxidant capacity, lipid peroxidation, RSSR to RSH ratio and the concentration of carbohydrates, glycogen, lipids and proteins in whole individuals (larvae or pupae) of Spodoptera exigua originating from two strains: control and selected over 120 generations with sublethal metal concentration (44 Cd mg per dry weight of diet). Generally, the increase of the protein, carbohydrates, glycogen concentration and lipid peroxidation decrease with age of the larvae were found. Revealed cases of a higher mobilisation of carbohydrates and proteins, and changes in total antioxidant capacity or lipid peroxidation, in individuals being under metal exposure, occurred in strain-depended mode. Short-term Cd exposure effect was connected with possible higher engagement of proteins and glycogen in detoxification processes, but also higher concentration of lipid peroxidation. In turn, for long-term Cd exposure effect lower lipids concentration and higher thiols usage seemed to be more specific.
镉作为一种常见的环境应激源，可能对草食性昆虫产生高度毒性作用。问题是，昆虫体内氧化应激和能量储备失衡的可能性是否取决于发育阶段、性别以及昆虫种群对镉的多代暴露史。因此，本研究的目的是比较源自两个品系（对照和经过 120 多代亚致死浓度金属（44Cdmg 每干重饮食）选择的品系）的甜菜夜蛾个体（幼虫或蛹）的发育特征、总抗氧化能力、脂质过氧化、RSSR 与 RSH 比值以及碳水化合物、糖原、脂质和蛋白质的浓度。总的来说，我们发现随着幼虫年龄的增长，蛋白质、碳水化合物、糖原浓度和脂质过氧化的增加。在受到金属暴露的个体中，发现了以菌株依赖模式发生的更高的碳水化合物和蛋白质动员以及总抗氧化能力或脂质过氧化变化的情况。短期 Cd 暴露的影响可能与蛋白质和糖原在解毒过程中可能的更高参与有关，但也与更高的脂质过氧化有关。相反，对于长期 Cd 暴露的影响，较低的脂质浓度和较高的硫醇利用似乎更为特殊。
