Wang Ning, Song Na, Tang Zejun, Wang Xiaojie, Kang Zhensheng, Dai Liangying, Wang Bing
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests and College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan, China.
Plants (Basel). 2020 Oct 6;9(10):1316. doi: 10.3390/plants9101316.
, as an effective model of cereal grains, is susceptible to most destructive cereal pathogens. () has been studied extensively in . is one of the important regulators of plant immunity. However, no homologous genes of were found in . In this study, the gene was transformed into Three transgenic plant lines containing the gene were confirmed by PCR and GUS gene activity. There were fewer urediospores in the -overexpressing plants compared to wild type plants. biomass was obviously decreased in transgenic plants. The length of infection hyphae and infection unit areas of were significantly limited in transgenic plants. Moreover, there were small lesions in transgenic plants challenged by . Salicylic acid accumulation was significantly increased, which led to elevated gene expression in transgenic inoculated by or compared to wild type plants. These results were consistent with infected phenotypes. Overexpression of in caused resistance to and . These results suggest that could regulate plant resistance in .
作为谷物的有效模型,[具体谷物名称]易受大多数具有破坏性的谷物病原体侵害。[相关研究对象]在[具体研究地点]已得到广泛研究。[相关基因名称]是植物免疫的重要调节因子之一。然而,在[具体物种名称]中未发现[相关基因名称]的同源基因。在本研究中,将[相关基因名称]基因转化到[具体植物名称]中。通过PCR和GUS基因活性鉴定出三个含有[相关基因名称]基因的转基因植物株系。与野生型植物相比,[相关基因名称]过表达植物中的[具体病原体名称]夏孢子较少。[具体植物名称]转基因植物中的[具体植物名称]生物量明显降低。在转基因植物中,[具体病原体名称]的侵染菌丝长度和侵染单位面积受到显著限制。此外,在受到[具体病原体名称]挑战的[具体植物名称]转基因植物中出现小的病斑。水杨酸积累显著增加,这导致与野生型植物相比,在接种[具体病原体名称]或[具体病原体名称]的转基因[具体植物名称]中[相关基因名称]基因表达升高。这些结果与感染表型一致。在[具体植物名称]中过表达[相关基因名称]导致对[具体病原体名称]和[具体病原体名称]具有抗性。这些结果表明,[相关基因名称]可以调节[具体植物名称]中的植物抗性。