Department of Dermatology, State University of New York, Downstate Health Sciences University, Brooklyn, New York, USA.
Dermatology Service, VA New York Harbor Healthcare System - Brooklyn Campus, Veterans hospital, Brooklyn, New York, USA.
J Biophotonics. 2021 Feb;14(2):e202000359. doi: 10.1002/jbio.202000359. Epub 2020 Nov 3.
Skin fibrosis is a debilitating feature of several systemic and dermatologic diseases. While current treatment options carry significant risk of side effects and recurrence, high-fluence light emitting diode-generated red light (LED-RL) is an alternative therapeutic that is safe, non-invasive, and accessible. We previously demonstrated LED-RL decreases fibroblast proliferation, a key pathogenic component of fibrosis. However, the cellular mechanism by which high fluence LED-RL modulates fibroblast proliferation is unclear. Herein, we explored the effects of high fluence LED-RL on human dermal fibroblast cell cycle progression. We demonstrate that LED-RL at 640 J/cm induced significant arrest of cells in G /G compared to temperature-matched control. This was accompanied by a corresponding increase in expression of checkpoint regulator p53 in irradiated cells. These data demonstrate high fluence LED-RL may exert its anti-proliferative effect on fibroblasts by inducing G /G arrest. Further, this study provides insight into the molecular mechanism underlying LED-RL as an anti-fibrotic therapeutic.
皮肤纤维化是几种系统性和皮肤科疾病的一种使人虚弱的特征。虽然目前的治疗选择有很大的副作用和复发风险，但高强度发光二极管产生的红光 (LED-RL) 是一种安全、非侵入性且易于获得的替代治疗方法。我们之前证明，LED-RL 可减少成纤维细胞增殖，这是纤维化的一个关键致病成分。然而，高强度 LED-RL 调节成纤维细胞增殖的细胞机制尚不清楚。在此，我们探讨了高强度 LED-RL 对人真皮成纤维细胞细胞周期进程的影响。我们证明，与温度匹配的对照相比，640 J/cm 的 LED-RL 可显著诱导细胞在 G1 期停滞。这伴随着照射细胞中检查点调节剂 p53 的表达相应增加。这些数据表明，高强度 LED-RL 通过诱导 G1 期停滞可能对成纤维细胞发挥其抗增殖作用。此外，这项研究为 LED-RL 作为一种抗纤维化治疗的分子机制提供了深入了解。
