Department of Biomedical and Surgical Sciences, Odontostomatological University Centre: Chair Prof. Stefano Cianetti, University of Perugia, Perugia, Italy.
Department of Experimental Medicine, Section of Biosciences and Medical Embryology, University of Perugia, Perugia, Italy.
Biomed Pharmacother. 2020 Dec;132:110728. doi: 10.1016/j.biopha.2020.110728. Epub 2020 Oct 7.
A systematic review was performed to evaluate the biological effects of Cannabidiol (CBD), one of the major components of Cannabis Sativa, on normal human healthy cell populations in terms of cell viability, proliferation, migration, apoptosis and inflammation. Inclusion criteria were: studies on cell lines and primary cell culture from healthy donors, CBD exposure as variable, no CBD exposure as control and published in English language. Quality assessment was based on ToxR tool, with a score of reliability ranging from 15 to 18.Following the PRISMA statement, three independent reviewers performed both a manual and an electronic search using MEDLINE via PubMed, Scopus, Web of Science and Cochrane. From a total of 9437eligible articles, 29 studies have been selected. The average quality assessment score was 16.48.Theresults showed heterogeneous CBD concentration exposure (0.01-50 μM or 0.1 nmol/mL-15 mg/mL). The definition of a threshold limit would allow the identification of specific effects on expected outcomes. From the data obtained CBD resulted to inhibit cell viability in a dose-dependent manner above 2 μM, while in oral cell populations the inhibitory concentration is higher than 10 μM. Moreover, it was observed a significantly inhibition of cell migration and proliferation. On the contrary, it was highlighted a stimulation of apoptosis only at high doses (from 10 μM).Finally, CBD produced an anti-inflammatory effect, with a reduction of the pro-inflammatory cytokine gene expression and secretion. CBD down-regulated ROS production, although at high concentrations (16 μM) increased ROS-related genes expression. The diffusion of CBD for therapeutic and recreational uses require a precise definition of its potential biological effects. A thorough knowledge of these aspects would allow a safe use of this substance without any possible side effects.
系统评价评估了大麻素（Cannabidiol，CBD）的生物学效应，CBD 是大麻中的主要成分之一，在细胞活力、增殖、迁移、凋亡和炎症方面对正常人类健康细胞群的影响。纳入标准为：来自健康供体的细胞系和原代细胞培养的研究、CBD 暴露作为变量、无 CBD 暴露作为对照以及以英文发表的研究。质量评估基于 ToxR 工具，可靠性评分为 15-18 分。根据 PRISMA 声明，三位独立审稿人使用 MEDLINE 通过 PubMed、Scopus、Web of Science 和 Cochrane 进行了手动和电子搜索。从总共 9437 篇合格文章中，选择了 29 项研究。平均质量评估得分为 16.48。结果显示，CBD 暴露浓度存在异质性（0.01-50 μM 或 0.1 nmol/mL-15 mg/mL）。阈值的定义可以确定对预期结果的特定影响。从获得的数据来看，CBD 在 2 μM 以上呈剂量依赖性方式抑制细胞活力，而在口腔细胞群中，抑制浓度高于 10 μM。此外，观察到细胞迁移和增殖显著受到抑制。相反，仅在高剂量（10 μM 以上）下观察到细胞凋亡受到刺激。最后，CBD 产生抗炎作用，降低促炎细胞因子的基因表达和分泌。CBD 下调 ROS 产生，尽管在高浓度（16 μM）时增加了与 ROS 相关的基因表达。为了治疗和娱乐目的而推广 CBD 需要对其潜在生物学效应进行精确定义。对这些方面的透彻了解将允许安全使用这种物质，而不会产生任何可能的副作用。
