麻（ L.）精油纳米乳液包封用于潜在治疗应用的研究：细胞毒性特征评估。

Encapsulation of Hemp ( L.) Essential Oils into Nanoemulsions for Potential Therapeutic Applications: Assessment of Cytotoxicological Profiles.

机构信息

Department of Experimental Medicine, School of Pharmacy, University of Camerino, Via Madonna delle Carceri 9, 62032 Camerino, Italy.

Chemistry Interdisciplinary Project (ChIP) Research Center, School of Pharmacy, University of Camerino, Via Madonna delle Carceri, 62032 Camerino, Italy.

出版信息

Molecules. 2023 Sep 7;28(18):6479. doi: 10.3390/molecules28186479.

DOI:10.3390/molecules28186479

PMID:37764255

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10537312/

Abstract

Industrial hemp ( L.), due to its bioactive compounds (terpenes and cannabinoids), has gained increasing interest in different fields, including for medical purposes. The evaluation of the safety profile of hemp essential oil (EO) and its encapsulated form (nanoemulsion, NE) is a relevant aspect for potential therapeutic applications. This study aimed to evaluate the toxicological effect of hemp EOs and NEs from cultivars Carmagnola CS and Uso 31 on three cell lines selected as models for topical and inhalant administration, by evaluating the cytotoxicity and the cytokine expression profiles. Results show that EOs and their NEs have comparable cytotoxicity, if considering the quantity of EO present in the NE. Moreover, cells treated with EOs and NEs showed, in most of the cases, lower levels of proinflammatory cytokines compared to Etoposide used as a positive control, and the basal level of inflammatory cytokines was not altered, suggesting a safety profile of hemp EOs and their NEs to support their use for medical applications.

摘要

工业大麻（L.）因其生物活性化合物（萜烯类和大麻素），在包括医疗用途在内的不同领域引起了越来越多的关注。大麻精油（EO）及其包封形式（纳米乳液，NE）的安全状况评估是潜在治疗应用的一个相关方面。本研究旨在评估卡玛格诺拉 CS 和乌索 31 品种的工业大麻 EO 和 NE 对三种细胞系的毒理学影响，这三种细胞系被选为局部和吸入给药的模型，通过评估细胞毒性和细胞因子表达谱。结果表明，如果考虑到 NE 中存在的 EO 量，EO 和它们的 NE 具有可比的细胞毒性。此外，与用作阳性对照的依托泊苷相比，用 EO 和 NE 处理的细胞在大多数情况下显示出较低水平的促炎细胞因子，并且炎症细胞因子的基础水平没有改变，这表明大麻 EO 及其 NE 具有安全性，支持将其用于医疗应用。