Kosukwatthana Phannicha, Rungsuriyawiboon Oumaporn, Rattanasrisomporn Jatuporn, Kimram Kanogwan, Tansakul Natthasit
Graduate Program in Animal Health and Biomedical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
Animals (Basel). 2024 Dec 20;14(24):3683. doi: 10.3390/ani14243683.
Cannabidiol, the primary non-psychoactive phytocannabinoid found in cannabis, has generated significant research interest due to its potential for biological effects, such as anti-inflammatory, analgesic, immunomodulatory, and anticonvulsant properties. Several studies have demonstrated the potential of CBD to alter inflammatory cytokines; however, data on CBD's effects on cell viability and pro-inflammatory cytokines in target animals, such as dogs, are limited. Therefore, in this study, we investigated the effects of CBD on the cell viability and modulation of pro-inflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α), in canine PBMCs stimulated with LPS. To evaluate the effect of CBD on neuroinflammation in epilepsy pathology, an independent study of five refractory epileptic dogs co-treated with CBD for 30 days was conducted. The current findings revealed that CBD concentrations of 16 µg/mL had a statistically significant effect on the viability of canine PBMCs with a calculated IC of 15.54 µg/mL. The effect of CBD on inflammatory cytokines in LPS-stimulated PBMCs tended to be dose-dependent, with CBD concentrations of 5-30 μg/mL resulting in decreased production of the tested pro-inflammatory cytokines. Considering the effect of CBD on cytokine production by PBMCs from epileptic dogs, CBD has the potential to modulate immune responses and provide benefits when used in combination with antiepileptic drugs. The findings provided evidence of CBD cytotoxicity and its effect on the alteration of pro-inflammatory cytokines in canine PBMCs.
大麻二酚是大麻中主要的非精神活性植物大麻素，因其具有抗炎、镇痛、免疫调节和抗惊厥等生物学效应的潜力而引起了广泛的研究关注。多项研究已证实大麻二酚有改变炎性细胞因子的潜力；然而，关于大麻二酚对犬等目标动物细胞活力和促炎细胞因子影响的数据有限。因此，在本研究中，我们调查了大麻二酚对脂多糖刺激的犬外周血单核细胞（PBMCs）中细胞活力以及白细胞介素（IL）-1β、IL-6和肿瘤坏死因子-α（TNF-α）等促炎细胞因子调节的影响。为评估大麻二酚在癫痫病理中对神经炎症的作用，我们对五只难治性癫痫犬进行了为期30天的联合大麻二酚治疗的独立研究。目前的研究结果显示，16μg/mL的大麻二酚浓度对犬PBMCs的活力有统计学显著影响，计算得出的半数抑制浓度（IC）为15.54μg/mL。大麻二酚对脂多糖刺激的PBMCs中炎性细胞因子的影响呈剂量依赖性，5 - 30μg/mL的大麻二酚浓度可导致所检测的促炎细胞因子产生减少。考虑到大麻二酚对癫痫犬PBMCs细胞因子产生的影响，大麻二酚有调节免疫反应的潜力，与抗癫痫药物联合使用时可能有益。这些发现为大麻二酚的细胞毒性及其对犬PBMCs中促炎细胞因子改变的影响提供了证据。
