Holt P G, Fimmel P J, Roberts L M, Keast D
Infect Immun. 1977 Jun;16(3):904-9. doi: 10.1128/iai.16.3.904-909.1977.
After inoculation with viable BCG organisms, spleen cells from mice developed cellular immune reactivity to purified protein derivative antigen by day 10, which declined by day 25; after rechallenge on day 25, a vigorous secondary response rapidly developed (by day 29). The inclusion of autochthonous serum (from the BCG-infected mice) in the in vitro cellular immune reactivity tests altered this pattern considerably: the primary response was shortened, and the secondary response totally abated, suggesting the presence of blocking factors active against BCG-specific cellular immune reactivity in the sera of infected animals. Subsequent experiments indicated that BCG-specific serum blocking factor activity could be generated in the serum of control mice by the intravenous inoculation of purified protein derivative.
用活卡介苗微生物接种后，小鼠的脾细胞在第10天时对纯化蛋白衍生物抗原产生细胞免疫反应性，到第25天时这种反应性下降；在第25天再次攻击后，迅速出现强烈的二次反应（到第29天）。在体外细胞免疫反应性试验中加入自身血清（来自感染卡介苗的小鼠），可使这种模式发生相当大的改变：初次反应缩短，二次反应完全消失，这表明感染动物血清中存在针对卡介苗特异性细胞免疫反应性的阻断因子。随后的实验表明，通过静脉接种纯化蛋白衍生物，可在对照小鼠的血清中产生卡介苗特异性血清阻断因子活性。
