Department of Microbiology, Fars Science and Research Branch, Islamic Azad University, Fars, Iran.
Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Cell Mol Biol (Noisy-le-grand). 2020 Jul 31;66(5):1-8.
MicroRNAs (miRNAs) as a small RNA and post-transcriptional modulator are shown to have regulatory effects for different cellular activities and pathways, such as metabolism, virus replication and also cell growth. In addition, miRNAs can regulate the replication of the hepatitis B virus (HBV). Therefore, the expression profile of miRNAs was evaluated in HBV infected patient groups and healthy controls. The expression levels of following microRNAs (as noninvasive biomarkers) were compared in two experimental (those with various stages of HBV infection) and control groups to evaluate their diagnosis ability: miR-122-5p, miR-125a-5p, miR-199a-3p, miR-210-5p, miR-205-5p, miR-155-5p, miR-372-5p, and miR-1-5p. RNA extraction was performed for 45 serum samples. The miRCURY LNA™ Universal RT-miRNA-PCR system and miRNA PCR panels were used for measuring microRNA expression profiles. To normalize quantitative values, the endogenous reference by UniSp6 expression was used.Serum miR-125a-5p and miR-122-5p were significantly higher in patients in different stages of HBV infection (p<0.001) than in controls (p<0.001). Receiver operating characteristic (ROC) curve analyses suggested that serum miR-125a-5p and miR-122-5p have significant diagnostic value for HBV infection. A significant difference was not found in terms of serum levels of other miRs (miR-199a-3p, miR-210-5p, miR-205-5p, miR-155-5p, miR-372-5p and miR-1-5p). Results suggest that miR-125a-5p and miR-122-5p can be used as possible noninvasive biomarkers for monitoring of HBV infection need to confirm in future completed studies.
微小 RNA（miRNAs）作为一种小 RNA 和转录后调节剂，被证明对不同的细胞活动和途径具有调节作用，如代谢、病毒复制和细胞生长。此外，miRNAs 可以调节乙型肝炎病毒（HBV）的复制。因此，评估了乙型肝炎病毒感染患者组和健康对照组中的 miRNA 表达谱。在两个实验组（感染 HBV 的不同阶段）和对照组中比较了以下 microRNAs（作为非侵入性生物标志物）的表达水平，以评估它们的诊断能力：miR-122-5p、miR-125a-5p、miR-199a-3p、miR-210-5p、miR-205-5p、miR-155-5p、miR-372-5p 和 miR-1-5p。从 45 个血清样本中提取 RNA。使用 miRCURY LNA™ Universal RT-miRNA-PCR 系统和 miRNA PCR 试剂盒测量 microRNA 表达谱。为了标准化定量值，使用内参 UniSp6 的表达进行归一化。在不同阶段的 HBV 感染患者的血清中 miR-125a-5p 和 miR-122-5p 明显高于对照组（p<0.001）。受试者工作特征（ROC）曲线分析表明，血清 miR-125a-5p 和 miR-122-5p 对乙型肝炎病毒感染具有显著的诊断价值。在血清中其他 microRNA（miR-199a-3p、miR-210-5p、miR-205-5p、miR-155-5p、miR-372-5p 和 miR-1-5p）的水平方面没有发现显著差异。结果表明，miR-125a-5p 和 miR-122-5p 可作为监测乙型肝炎病毒感染的潜在非侵入性生物标志物，但需要在未来的研究中进一步验证。
