Facultad de Medicina Clínica Alemana Universidad del Desarrollo, Programa Hantavirus, Instituto de Ciencias e Innovación en Medicina, Santiago, Chile.
Division of Immunobiology, Department of Medicine, University of Vermont, Burlington, VT, United States.
Front Cell Infect Microbiol. 2020 Sep 17;10:444. doi: 10.3389/fcimb.2020.00444. eCollection 2020.
(ANDV) is the etiologic agent of hantavirus cardiopulmonary syndrome (HCPS), which has a case fatality rate around 35%, with no effective treatment or vaccine available. ANDV neutralizing antibody (NAb) measurements are important for the evaluation of the immune response following infection, vaccination, or passive administration of investigational monoclonal or polyclonal antibodies. The standard assay for NAb measurement is a focus reduction neutralization test (FRNT) featuring live ANDV and must be completed under biosafety level (BSL)-3 conditions. In this study, we compared neutralization assays featuring infectious ANDV or vesicular stomatitis virus (VSV) pseudovirions decorated with ANDV glycoproteins for their ability to measure anti-ANDV NAbs from patient samples. Our studies demonstrate that VSV pseudovirions effectively measure NAb from clinical samples and have greater sensitivity compared to FRNT with live ANDV. Importantly, the pseudovirus assay requires less labor and sample materials and can be conducted at BSL-2.
（ANDV）是汉坦病毒心肺综合征（HCPS）的病原体，其病死率约为 35%，目前尚无有效的治疗方法或疫苗。抗 ANDV 中和抗体（NAb）的测量对于评估感染、接种疫苗或被动给予研究性单克隆或多克隆抗体后的免疫反应非常重要。NAb 测量的标准检测方法是使用活 ANDV 的蚀斑减少中和试验（FRNT），并且必须在生物安全级别（BSL）-3 条件下完成。在这项研究中，我们比较了具有传染性 ANDV 或带有 ANDV 糖蛋白的水疱性口炎病毒（VSV）假病毒的中和测定，以评估其测量来自患者样本的抗 ANDV NAb 的能力。我们的研究表明，VSV 假病毒可有效测量临床样本中的 NAb，与使用活 ANDV 的 FRNT 相比，其灵敏度更高。重要的是，假病毒测定所需的劳动力和样本材料更少，并且可以在 BSL-2 下进行。
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